Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil.
Parasitology. 2013 Jan;140(1):69-75. doi: 10.1017/S0031182012001230. Epub 2012 Aug 29.
The aim of this study was to fractionate and partially characterize the antigenic extract of filariform larvae of Strongyloides venezuelensis in ion-exchange resin diethylaminoethyl sepharose (DEAE), to obtain antigenic fractions potentially applicable in immunoassays. Somatic antigen (SA) and its fractions DEAE S1 and DEAE S2 - which interacted with the resin - were evaluated by 1-dimensional electrophoresis to obtain protein profiles. SA and its fractions were tested in serum samples for IgG detection by ELISA. Serum samples (n = 155) were analysed: 50 from strongyloidiasis patients (G1), 55 from patients with other parasitic infections (G2) and 50 from healthy volunteers. Sensitivity (Se), specificity (Sp), area under curve (AUC) and likelihood ratios (LR) were calculated. The DEAE S2 fraction provided a high diagnostic value for IgG detection (Se 92·0%, Sp 91·4%, AUC 0·981, LR+ 10·75, LR - 0·09). In conclusion, the DEAE S2 fraction would probably be a source of immunodominant polypeptides for IgG detection in human strongyloidiasis serodiagnosis.
本研究的目的是通过离子交换树脂 DEAE-纤维素对委内瑞拉旋毛线虫丝状幼虫的抗原提取物进行分离和部分鉴定，以获得潜在适用于免疫分析的抗原部分。通过 1 维电泳评估体细胞抗原 (SA)及其与树脂相互作用的 DEAE S1 和 DEAE S2 部分，以获得蛋白质图谱。通过 ELISA 检测 SA 及其部分在血清样本中 IgG 的检测。分析了 155 份血清样本：50 份来自旋毛虫病患者 (G1)，55 份来自其他寄生虫感染患者 (G2)，50 份来自健康志愿者。计算了灵敏度 (Se)、特异性 (Sp)、曲线下面积 (AUC) 和似然比 (LR)。DEAE S2 部分对 IgG 检测具有较高的诊断价值 (Se 92.0%，Sp 91.4%，AUC 0.981，LR+ 10.75，LR - 0.09)。总之，DEAE S2 部分可能是旋毛虫病血清学诊断中 IgG 检测的免疫优势多肽的来源。
