采用离子交换层析法获得的带绦虫囊尾蚴抗原性片段：适用于人类脑囊尾蚴病免疫诊断的免疫优势标志物的潜在来源。
Taenia saginata metacestode antigenic fractions obtained by ion-exchange chromatography: potential source of immunodominant markers applicable in the immunodiagnosis of human neurocysticercosis.
机构信息
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, 38400-902, Uberlândia, MG, Brazil.
出版信息
Diagn Microbiol Infect Dis. 2013 May;76(1):36-41. doi: 10.1016/j.diagmicrobio.2013.01.020. Epub 2013 Feb 19.
The aim of this study was to fractionate and partially characterize fractions obtained from the total saline extract (SE) of Taenia saginata metacestodes after ion-exchange procedure in carboxymethyl sepharose (CM) and diethylaminoethyl sepharose (DEAE) resins, as a source of antigenic markers applicable in the immunodiagnosis of neurocysticercosis (NCC). For IgG detection by enzyme-linked immunosorbent assay (ELISA) and immunoblotting, 140 serum samples were analyzed: 45 from patients with NCC (G1), 50 from patients with other parasitic infections (G2), and 45 from healthy individuals. Sensitivity (Se), specificity (Sp), area under curve (AUC), and likelihood ratios (LR) were calculated. CM S2 and DEAE S2 fractions provided high diagnostic values (Se 88.8% and 93.4%; Sp 93.7% and 92.6%; AUC 0.965 and 0.987; LR+ 14.07 and 12.67; LR- 0.11 and 0.07, respectively). In conclusion, CM S2 and DEAE S2 fractions are important sources of specific peptides, with high efficiency to diagnose NCC.
本研究的目的是对离子交换羧甲基琼脂糖（CM）和二乙氨基乙基琼脂糖（DEAE）树脂后从猪带绦虫囊尾蚴总盐提取物（SE）中获得的部分进行分离和部分特性描述，作为抗原标记物的来源适用于神经囊尾蚴病（NCC）的免疫诊断。通过酶联免疫吸附试验（ELISA）和免疫印迹法检测 IgG，分析了 140 份血清样本：45 份来自 NCC 患者（G1），50 份来自其他寄生虫感染患者（G2），45 份来自健康个体。计算了灵敏度（Se）、特异性（Sp）、曲线下面积（AUC）和似然比（LR）。CM S2 和 DEAE S2 馏分提供了高诊断价值（Se 88.8%和 93.4%；Sp 93.7%和 92.6%；AUC 0.965 和 0.987；LR+ 14.07 和 12.67；LR- 0.11 和 0.07）。总之，CM S2 和 DEAE S2 馏分是具有高效诊断 NCC 的特异性肽的重要来源。
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