Department of Gastroenterology, Second Affiliated Hospital, Fujian Medical University, Quanzhou, Fujian 362000, China.
Chin Med J (Engl). 2012 Sep;125(17):3161-5.
CD44v6 plays an important role in invasion and metastasis of tumor, Livin has anti-apoptotic effects. The present study aimed to explore the expression and clinical significance of CD44v6 and Livin in gastric cancer tissue.
Streptavidin-peroxidase linked immunohistochemical method was used to determine the expression of CD44v6 and Livin in gastric cancer tissue and adjacent normal gastric tissues from 59 patients with histopathologically confirmed gastric cancer, and in gastric tissue specimens of 15 patients with gastric polyps, and 15 patients with chronic non-atrophic gastritis. The chi-square test was used for comparison of the relevant factors, Spearman's rank correlation test was applied for relationship among positive expression of the proteins.
The expresion of CD44v6 was positive in 64.4% of the gastric cancer patients; 5.1%, 0 and 13.3% in specimens of normal tissues adjacent to the cancer tissues, in gastric tissue specimens of patients with gastric polyps, and patients with chronic non-atrophic gastritis, respectively. The expression of Livin was positive in 52.5% of the gastric cancer tissues, 6.8%, 0 and 6.7% in the adjacent normal gastric tissue, specimens of patients with gastric polyps and chronic non-atrophic gastritis, respectively. The expression of CD44v6 was significantly correlated with the depth of invasion, the degree of differentiation, and lymphnode metastasis of gastric cancer (P < 0.05). The positive expression rate of Livin protein was also significantly correlated with degree of differentiation of gastric cancer cells and metastasis to lymphnodes (P < 0.05), but not correlated with the depth of invasion and pathological types (P > 0.05). The expression of CD44v6 and Livin in the gastric cancer tissue was positively correlated (r(s) = 0.286, P = 0.028).
The increased expression of CD44v6 and Livin in gastric cancer tissue may be closely related with development and progression of gastric cancer. CD44v6 and Livin may be new biological markers of gastric cancer.
CD44v6 在肿瘤的侵袭和转移中发挥重要作用，Livin 具有抗凋亡作用。本研究旨在探讨 CD44v6 和 Livin 在胃癌组织中的表达及临床意义。
采用链霉亲和素-过氧化物酶连接免疫组织化学法检测 59 例经组织病理学证实的胃癌患者的胃癌组织及癌旁正常胃组织、15 例胃息肉患者和 15 例慢性非萎缩性胃炎患者的胃组织标本中 CD44v6 和 Livin 的表达，采用卡方检验比较相关因素，Spearman 秩相关检验分析蛋白阳性表达的关系。
64.4%的胃癌患者 CD44v6 表达阳性；癌旁正常组织、胃息肉患者和慢性非萎缩性胃炎患者的表达率分别为 5.1%、0 和 13.3%。52.5%的胃癌组织 Livin 表达阳性，癌旁正常组织、胃息肉患者和慢性非萎缩性胃炎患者的表达率分别为 6.8%、0 和 6.7%。CD44v6 的表达与胃癌的浸润深度、分化程度和淋巴结转移显著相关（P < 0.05）。Livin 蛋白的阳性表达率与胃癌细胞的分化程度和淋巴结转移也显著相关（P < 0.05），但与浸润深度和病理类型无关（P > 0.05）。胃癌组织中 CD44v6 和 Livin 的表达呈正相关（r(s) = 0.286，P = 0.028）。
胃癌组织中 CD44v6 和 Livin 的表达增加可能与胃癌的发生发展密切相关。CD44v6 和 Livin 可能是胃癌的新的生物学标志物。
