State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China.
Biotechnol Lett. 2012 Dec;34(12):2291-8. doi: 10.1007/s10529-012-1035-z. Epub 2012 Aug 30.
The antifungal properties and mechanism of three types of chitosan against the rice sheath blight pathogen, Rhizoctonia solani, were evaluated. Each chitosan had strong antifungal activity against R. solani and protected rice seedlings from sheath blight, in particular, two types of acid-soluble chitosan caused a 60-91 % inhibition in mycelial growth, 31-84 % inhibition of disease incidence, and 66-91 % inhibition in lesion length. The mechanism of chitosan in protection of rice from R. solani pathogen was attributed to direct destruction of the mycelium, evidenced by scanning and transmission electron microscopic observations and pathogenicity testing; indirect induced resistance was evidenced by the changes in the activities of the defense-related phenylalanine ammonia lyase, peroxidase and polyphenol oxidase in rice seedling. To our knowledge, this is the first report on the antifungal activity of chitosan against rice R. solani.
评价了三种壳聚糖对水稻纹枯病菌（Rhizoctonia solani）的抑菌特性和作用机制。三种壳聚糖均对 R. solani 具有较强的抑菌活性，能有效保护水稻幼苗免受纹枯病侵害，其中两种酸溶性壳聚糖对菌丝生长的抑制率为 60-91%，发病率抑制率为 31-84%，病斑长度抑制率为 66-91%。壳聚糖保护水稻免受 R. solani 病原菌侵害的机制归因于对菌丝的直接破坏，扫描和透射电子显微镜观察和致病性试验证实了这一点；间接诱导抗性的证据是水稻幼苗中防御相关苯丙氨酸解氨酶、过氧化物酶和多酚氧化酶活性的变化。据我们所知，这是壳聚糖对水稻 R. solani 抑菌活性的首次报道。
