Fiehn N E, Westergaard J
Department of Microbiology, Royal Dental College, Copenhagen, Denmark.
Scand J Dent Res. 1990 Oct;98(5):412-21. doi: 10.1111/j.1600-0722.1990.tb00992.x.
Different bacterial profiles of subgingival plaque have been described for different clinical categories of marginal periodontitis. In the present investigation the subgingival microflora was studied in young adults with advanced marginal periodontitis. From 12 patients pooled subgingival plaque samples from 5 advanced stages of diseased sites were examined by direct differential phase-contrast microscopy and by cultivation on enriched and selective media. The proportions of the following genera and species were calculated: black-pigmented Bacteroides sp., B. gingivalis, Fusobacterium nucleatum, Veillonella sp., Actinobacillus actinomycetemcomitans, Capnocytophaga sp., Eikenella corrodens, Campylobacter sp., and Candida sp. Spirochete identification was carried out ultrastructurally. Calculated percent minimum similarity levels between the individuals revealed that each patient harbored its characteristic cultivable subgingival microflora different from the other individuals. The spirochetes seemed to constitute the subgingival bacterial group with the smallest variation as certain morphotypes were dominating in almost all individuals.
对于边缘性牙周炎的不同临床分类，已描述了龈下菌斑的不同细菌谱。在本研究中，对患有晚期边缘性牙周炎的年轻成年人的龈下微生物群进行了研究。从12名患者中，采集了来自患病部位5个晚期阶段的混合龈下菌斑样本，通过直接相差显微镜检查以及在富集和选择性培养基上培养进行检测。计算了以下属和种的比例：产黑色素类杆菌属、牙龈类杆菌、具核梭杆菌、韦荣氏菌属、伴放线放线杆菌、二氧化碳嗜纤维菌属、腐蚀埃肯菌、弯曲菌属和念珠菌属。通过超微结构进行螺旋体鉴定。个体之间计算出的最低相似性百分比水平表明，每个患者都有其独特的可培养龈下微生物群，与其他个体不同。螺旋体似乎构成了龈下细菌组中变异最小的菌群，因为某些形态型在几乎所有个体中都占主导地位。
