López N J, Mellado J C, Giglio M S, Leighton G X
Department of Periodontics, Faculty of Dentistry, University of Chile, Santiago.
J Periodontol. 1995 Jul;66(7):559-67. doi: 10.1902/jop.1995.66.7.559.
The occurrence of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Eikenella corrodens, Fusobacterium nucleatum, Campylobacter rectus, Capnocytophaga species, and certain bacterial morphotypes was determined in 18 affected and 18 unaffected sites in 10 localized juvenile periodontitis (LJP) patients, and in 10 affected and 10 unaffected sites in 5 generalized juvenile periodontitis (GJP) patients. The subgingival proportion of the 7 bacterial species was determined by selective and nonselective culturing. The results showed that when considering the pure prevalence of bacteria ( > 0%) there were significant differences (P < 0.05) in the subgingival plaque microflora of the affected sites versus those of the unaffected sites for P. gingivalis, A. actinomycetemcomitans, P. intermedia, E. corrodens, C. rectus, and F. nucleatum in LJP, and for P. gingivalis, P. intermedia, and F. nucleatum in GJP. The mean proportions of cocci, motile rods and spirochetes were also significantly different (P < 0.05) in affected sites compared to unaffected sites. Capnocytophaga sp, F. nucleatum, P. intermedia, and E. corrodens were found in more than 75% of affected sites in LJP. When taking the approach that an organism, to be associated with periodontal disease, has to be detected above a certain minimum threshold, the results indicated that bacteria most frequently associated with LJP and GJP in Chile are P. gingivalis (66% of LJP and 80% of GJP affected sites), and A. actinomycetemcomitans (44% of LJP and 50% in GJP affected sites). Different bacterial species may be judged to be important in the disease process depending upon whether a pure bacterial prevalence, or a prevalence above a certain detection level, is considered.
在10例局限性青少年牙周炎（LJP）患者的18个患牙部位和18个未患牙部位，以及5例广泛性青少年牙周炎（GJP）患者的10个患牙部位和10个未患牙部位，检测了伴放线放线杆菌、牙龈卟啉单胞菌、中间普氏菌、腐蚀埃肯菌、具核梭杆菌、直肠弯曲菌、二氧化碳嗜纤维菌属以及某些细菌形态型的存在情况。通过选择性和非选择性培养确定了7种细菌的龈下比例。结果显示，就细菌的单纯患病率（>0%）而言，LJP中，患牙部位与未患牙部位的龈下菌斑微生物群在牙龈卟啉单胞菌、伴放线放线杆菌、中间普氏菌、腐蚀埃肯菌、直肠弯曲菌和具核梭杆菌方面存在显著差异（P<0.05）；GJP中，患牙部位与未患牙部位在牙龈卟啉单胞菌、中间普氏菌和具核梭杆菌方面存在显著差异（P<0.05）。与未患牙部位相比，患牙部位球菌、运动杆菌和螺旋体的平均比例也存在显著差异（P<0.05）。在LJP超过75%的患牙部位发现了二氧化碳嗜纤维菌属、具核梭杆菌、中间普氏菌和腐蚀埃肯菌。当采用一种生物体若要与牙周疾病相关联就必须在某个最低阈值以上被检测到的方法时，结果表明，在智利，与LJP和GJP最常相关的细菌是牙龈卟啉单胞菌（LJP患牙部位的66%和GJP患牙部位的80%）以及伴放线放线杆菌（LJP患牙部位的44%和GJP患牙部位的50%）。根据是考虑单纯细菌患病率还是某个检测水平以上的患病率，不同的细菌种类在疾病过程中可能被判定为重要。
