Beck O, Boreus L O, Borg S, Jacobsson G, Lafolie P, Stensiö M
Department of Clinical Pharmacology, Karolinska Hospital, Stockholm, Sweden.
Ther Drug Monit. 1990 Sep;12(5):473-7.
Determination of plasma methadone is essential in connection with dose adjustments for patients participating in methadone maintenance programs. We successfully adapted the existing fluorescence polarization immunoassay (FPIA) kit intended for urinary methadone to plasma assays. A concentration interval of 50-900 ng/ml could be covered. The coefficient of variation was less than 7%, and the limit of detection below 50 ng/ml. The intercorrelation between the immunoassay and a specific gas chromatographic-mass spectrometric (GC-MS) method was studied in samples from 19 heroin addicts in methadone maintenance treatment. A total number of 97 plasma samples with a concentration range of 31-842 ng/ml were used. The slope and intercept of the regression line (CFPIA = 0.93 X CGC-MS + 15) was in good agreement with the theoretical relation (CFPIA = CGC-MS), with a coefficient of correlation of 0.978. The mean ratio, in quantitative result, between the techniques (CFPIA/CGC-MS) was 1.03 +/- 0.01 (SEM). We conclude that the immunoassay proposed in this study can be safely used in patients participating in methadone maintenance programs.
对于参与美沙酮维持治疗项目的患者进行剂量调整时，测定血浆美沙酮至关重要。我们成功地将现有的用于尿样美沙酮检测的荧光偏振免疫分析（FPIA）试剂盒适配于血浆检测。该方法可覆盖50 - 900 ng/ml的浓度范围。变异系数小于7%，检测限低于50 ng/ml。在19名接受美沙酮维持治疗的海洛因成瘾者的样本中，研究了免疫分析方法与特定气相色谱 - 质谱联用（GC - MS）方法之间的相互关系。总共使用了97份浓度范围为31 - 842 ng/ml的血浆样本。回归线的斜率和截距（CFPIA = 0.93×CGC - MS + 15）与理论关系（CFPIA = CGC - MS）高度吻合，相关系数为0.978。两种技术定量结果的平均比值（CFPIA/CGC - MS）为1.03±0.01（标准误）。我们得出结论，本研究中提出的免疫分析方法可安全地用于参与美沙酮维持治疗项目的患者。
