Zhang Wei, Chen Ming-Ren, Xiong Jun
The Affiliated Hospital of Jiangxi College of Chinese Medicine, Nanchang 330006, China.
Zhen Ci Yan Jiu. 2012 Jun;37(3):202-5, 217.
To observe the effects of different quantity of moxibustion of "Dazhui" (GV 14)on the levels of serum interleukin-4 (IL-4), interferon-gamma (IFN-gamma), and immunoglobulin E (IgE) in asthma rats, so as to reveal its mechanisms underlying immune regulation.
Sixty male SD rats were randomly divided into control, model, moxibustion-15 min (M-15 min), M-30 min, M-60 min, and M-120 min groups (10 rats/group). Asthma model was established by intraperitoneal injection of sensitized-suspension (containing egg albumin, aluminium hydroxide, and deactivated Bacillus pertussis) and ovalbumin-aerosol inhalation. Moxibustion was applied to "Dazhui" (GV 14) for 15, 30, 60 and 120 min, respectively. Serum IL-4, IFN-gamma and IgE contents were assayed by ELISA (enzyme-linked immunosorbent assay).
Compared with the control group, serum IL-4, IFN-gamma and IgE levels were increased significantly (P<0.01), and serum IFN-gamma/IL-4 level was down-regulated obviously (P<0.01)in the model group. After the moxibustion treatment, the levels of serum IL-4 and IgE in the M-15 min, M-30 min, M-60 min, and M-120 min groups were reduced significantly compared with the model group (P<0.01, P<0.05). The ratios of IFN-gamma/IL-4 in the M-15 min, M-30 min, M-60 min, and M-120 min groups were significantly higher than that in the model group (P<0.05, P<0.01). Comparison among the M-15 min, M-30 min, M-60 min, and M-120 min groups showed that the therapeutic effects of the M-30 min group were evidently superior to those of the M-15 min group, and those of the M-60 min group were significantly superior to those of the M-30 min group in lowering serum IL-4 and IgE contents and up-regulating IFN-gamma/IL-4 (P<0.05, P<0.01). No significant differences were found between the M-60 min and M-120 min groups in down-regulating IL-4 and IgE levels, and in raising IFN-gamma/IL-4 level, as well as among the M-15min, M-30 min, M-60 min and M-120 min groups in elevating serum IFN-gamma levels (P>0.05).
Moxibustion can restrain the secretion of IL-4, relieve the inflammatory reaction of IgE, and enhance the ratio of IFN-gamma/IL-4 in asthma rats, which presents an increase tendency of the curative effect along with the extension of the treatment time from 15 min to 60 min. Excessively long time (>60 min)of treatment is not necessary.
观察不同灸量的“大椎”（GV 14）对哮喘大鼠血清白细胞介素-4（IL-4）、干扰素-γ（IFN-γ）及免疫球蛋白E（IgE）水平的影响，以揭示其免疫调节机制。
将60只雄性SD大鼠随机分为对照组、模型组、艾灸15分钟（M-15分钟）组、M-30分钟组、M-60分钟组和M-120分钟组（每组10只）。通过腹腔注射致敏混悬液（含卵白蛋白、氢氧化铝和灭活百日咳杆菌）及雾化吸入卵白蛋白建立哮喘模型。分别对“大椎”（GV 14）进行15、30、60和120分钟的艾灸。采用酶联免疫吸附测定法（ELISA）检测血清IL-4、IFN-γ和IgE含量。
与对照组比较，模型组血清IL-4、IFN-γ和IgE水平显著升高（P<0.01），血清IFN-γ/IL-4水平明显下调（P<0.01）。艾灸治疗后，M-15分钟组、M-30分钟组、M-60分钟组和M-120分钟组血清IL-4和IgE水平较模型组显著降低（P<0.01，P<0.05）；M-15分钟组、M-30分钟组、M-60分钟组和M-120分钟组IFN-γ/IL-4比值显著高于模型组（P<0.05,P<0.01）。M-15分钟组、M-30分钟组、M-60分钟组和M-120分钟组比较结果显示M-30分钟组降低血清IL-4和IgE含量及上调IFN-γ/IL-γ比值的疗效明显优于M-15分钟组，M-60分钟组显著优于M-30分钟组（P<0.05，P<0.01）。M-60分钟组与M-120分钟组在下调IL-4和IgE水平、上调IFN-γ/IL-4比值方面差异无统计学意义，M-15分钟组、M-30分钟组、M-60分钟组和M-120分钟组在升高血清IFN-γ水平方面差异无统计学意义（P>0.05）。
艾灸可抑制哮喘大鼠IL-4的分泌，减轻IgE的炎症反应，提高IFN-γ/IL-4比值，且随着治疗时间从15分钟延长至60分钟疗效呈增加趋势，治疗时间过长（>60分钟）无必要。
