Chen Xiang-Hua, He Hai-Ming
Clinical College of Acupuncture-moxibustion and Orthopedic, Anhui University of Chinese Medicine, Hefei 230038, China.
Zhen Ci Yan Jiu. 2017 Apr 25;42(2):159-62.
To observe the effect of moxibustion at "Feishu" (BL 13) and "Shenshu"(BL 23) on peripheral blood T cells and serum interleukin in rats with asthma, so as to explore its immunological mechanisms in relieving asthma.
Thirty SD rats were randomly divided into normal, model and moxibustion groups(10 rats/group). Asthma model was established by intraperitoneal injection of ovalbumin and aluminum hydroxide, and ovalbumin inhalation. Moxibustion was applied to bilateral "Feishu" (BL 13) and "Shenshu"(BL 23) for 30 min, once daily for 14 d. The pathologic changes of the lung tissue were detected by H.E. staining. The levels of CD 3, CD 4, CD 8 T cells in whole blood were detected by flow cytometry. Serum IgE, IL-1 β, IL-1 Ra contents were assayed by ELISA (enzyme-linked immunosorbent assay).
The thickened muscle of bronchial wall, mucosal edema, infiltration of inflammatory cells and partial destruction of the alveolar structure were found in the model group. While in the moxibustion group, the structure of the alveolar wall was complete, the morphology of the bronchioles was regular, and there were no mucus plug formation and epithelial cell shedding in the lumen. The contents of blood CD 8 and CD 3 T cells, and serum IgE and IL-1 β in the model group were obviously higher than those in the normal group (<0.01). Compared with the model group, the levels of blood CD 8 T cell, serum IgE and IL-1 β in the moxibustion group were all decreased (<0.05,<0.01), meanwhile the levels of blood CD 4 T cell and serum IL-1 Ra were increased (<0.01,<0.05).
Moxibustion can reduce the levels of serum IgE and IL-1 β, and increase the level of serum IL-1 Ra, which may play an important role in the treatment of asthma.
观察艾灸“肺俞”（BL 13）和“肾俞”（BL 23）对哮喘大鼠外周血T细胞及血清白细胞介素的影响，以探讨其缓解哮喘的免疫机制。
将30只SD大鼠随机分为正常组、模型组和艾灸组（每组10只）。采用腹腔注射卵白蛋白和氢氧化铝，以及雾化吸入卵白蛋白的方法建立哮喘模型。对双侧“肺俞”（BL 13）和“肾俞”（BL 23）进行艾灸，每次30分钟，每日1次，共14天。采用苏木精-伊红（H.E.）染色法检测肺组织病理变化。采用流式细胞术检测全血中CD 3、CD 4、CD 8 T细胞水平。采用酶联免疫吸附测定法（ELISA）检测血清免疫球蛋白E（IgE）、白细胞介素-1β（IL-1β）、白细胞介素-1受体拮抗剂（IL-1Ra）含量。
模型组可见支气管壁肌层增厚、黏膜水肿、炎症细胞浸润及部分肺泡结构破坏。艾灸组肺泡壁结构完整，细支气管形态规则，管腔内无黏液栓形成及上皮细胞脱落。模型组血中CD 8、CD 3 T细胞及血清IgE、IL-1β含量明显高于正常组（P<0.01）。与模型组比较，艾灸组血中CD 8 T细胞、血清IgE及IL-1β水平均降低（P<0.05，P<0.01），同时血中CD 4 T细胞及血清IL-1Ra水平升高（P<0.01，P<0.05）。
艾灸可降低血清IgE及IL-1β水平，升高血清IL-1Ra水平，这可能在哮喘治疗中发挥重要作用。
