Laboratory of Pharmacology, Faculty of Medicine, University of Monastir, 5019 Monastir, Tunisia.
Nat Prod Res. 2013;27(12):1075-83. doi: 10.1080/14786419.2012.717284. Epub 2012 Aug 30.
This study investigates the antiulcerogenic and antibacterial activities of Apium graveolens extracts. The antiulcerogenic activity was evaluated in rats by the HCl/EtOH method. Inhibition of gastric lesions by A. graveolens extracts was dose-dependent for both aerial part (53-76%) and seeds (51-95%). The methanolic extract as well as the aqueous extracts used at 300 mg kg(-1) dose exhibited a highly significant inhibition of gastric lesions (91% and 95%, respectively) which was similar to that induced by omeprazole (94%). Essential oil and aqueous extract prepared from the aerial parts of A. graveolens were tested to determine their antibacterial activity using the paper disc-diffusion method, the minimal inhibitory concentration and the minimal bactericidal concentration. Essential oil of A. graveolens was strongly inhibitory against Escherichia coli and moderately inhibitory against Pseudomonas aeruginosa and Staphylococcus aureus. The chemical composition of the volatile oil was investigated by gas chromatography analysis. The major components identified were β-pinene, camphene, cumene, limonene, α-thuyene, α-pinene, β-phellendrene, p-cymene, γ-terpinene, sabinene and terpinolene.
本研究考察了芹菜提取物的抗溃疡和抗菌活性。采用 HCl/EtOH 法在大鼠中评估抗溃疡活性。芹菜地上部分（53-76%）和种子（51-95%）提取物对胃损伤的抑制作用呈剂量依赖性。在 300mg/kg 剂量下，甲醇提取物和水提取物均表现出对胃损伤的高度显著抑制作用（分别为 91%和 95%），与奥美拉唑（94%）诱导的作用相似。使用纸片扩散法、最小抑菌浓度和最小杀菌浓度测试了从芹菜地上部分制备的精油和水提取物的抗菌活性。芹菜精油对大肠杆菌具有很强的抑制作用，对铜绿假单胞菌和金黄色葡萄球菌具有中度抑制作用。通过气相色谱分析研究了挥发油的化学成分。鉴定的主要成分有β-蒎烯、莰烯、枯烯、柠檬烯、α-葑烯、α-蒎烯、β-水芹烯、对伞花烃、γ-萜品烯、月桂烯和松油醇。
