Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang 310029, China.
ACS Nano. 2012 Sep 25;6(9):7634-43. doi: 10.1021/nn3034056. Epub 2012 Aug 31.
Approximately 88% of the world population lives in regions with intermediate to high incidence of Hepatitis B virus (HBV), yet current serological and DNA-based detection methods have limited sensitivity and convenience. Here, we describe a preassembled plasmonic resonance nanocluster for HBV detection. The gold nanoparticle acceptors (AuNPs), with HBV surface antigen (HBsAg) epitope, and quantum dot (QD) donors with Fab antibody, are assembled into an immuno-mediated 3D-oriented complex with enhanced energy transfer and fluorescence quenching. The coherent plasmonic resonance between Au and QD nanoparticles is exploited to achieve improved donor-acceptor resonance within the nanocluster, which in the presence of HBV viral particles is disassembled in a highly specific manner. The nanocluster provides high detection specificity and sensitivity of HBV, with a sensitivity limit down to 1-100 viral particles per microliter and to attomolar levels of HBsAg. This general platform could be used to establish multiplex diagnostic assays for a variety of other microbial pathogens.
世界上约有 88%的人口生活在乙型肝炎病毒（HBV）发病率中等偏高的地区，但目前基于血清学和 DNA 的检测方法灵敏度和便利性有限。在这里，我们描述了一种用于 HBV 检测的预组装等离子体共振纳米簇。金纳米颗粒受体（AuNPs）带有 HBV 表面抗原（HBsAg）表位，而量子点（QD）供体带有 Fab 抗体，它们组装成一个免疫介导的 3D 定向复合物，增强了能量转移和荧光猝灭。Au 和 QD 纳米颗粒之间的相干等离子体共振被利用来实现纳米簇内供体-受体的共振改进，在存在 HBV 病毒颗粒的情况下，纳米簇以高度特异性的方式解组装。该纳米簇对 HBV 具有高检测特异性和灵敏度，检测下限低至每微升 1-100 个病毒颗粒，对 HBsAg 的检测下限低至飞摩尔水平。这个通用平台可以用于建立用于多种其他微生物病原体的多重诊断检测。
