Cancer Registry of Norway, Institute of Population-based Cancer Research, N-0310 Oslo, Norway.
Scand J Clin Lab Invest. 2012 Nov;72(7):555-62. doi: 10.3109/00365513.2012.705888. Epub 2012 Aug 30.
To investigate testosterone stability in archival serum samples stored for etiological cancer research, and compare two methods for testosterone measurements.
Four sets of 130 serum samples were randomly selected from male blood donors, aged 40-49 years at the time of blood draw. The sets had been stored at -25°C for 1 month, 4, 17 and 29 years, respectively, and were analyzed for testosterone, sex hormone binding globuline, follicle stimulating hormone, luteinizing hormone, sodium, albumin and cotinine. Testosterone was measured by two methods, an electrochemiluminescence immunoassay, and a liquid chromatography tandem mass spectrometry method.
The mean level of testosterone in the samples with the longest storage time (29 years) was substantially higher than that of the fresh samples. The two techniques gave approximately equal results for testosterone values in the range 5-27.5 nmol/L, close to normal range.
The high mean levels of testosterone in the oldest samples suggest a downward trend over the last three decades, as any degradation during storage would tend to give the opposite result. For archival serum samples, both electrochemiluminescence immunoassay and liquid chromatography tandem mass spectrometry are applicable methods for measurement of testosterone within the expected reference range.
研究用于癌症病因学研究的存档血清样本中睾酮的稳定性，并比较两种睾酮测量方法。
从年龄在采血时为 40-49 岁的男性献血者中随机选择了四组 130 份血清样本。这些样本分别在-25°C 下储存了 1 个月、4 年、17 年和 29 年，并分析了睾酮、性激素结合球蛋白、卵泡刺激素、黄体生成素、钠、白蛋白和可替宁。睾酮采用两种方法进行测量，电化学发光免疫分析法和液相色谱串联质谱法。
储存时间最长（29 年）的样本中的睾酮平均水平明显高于新鲜样本。对于 5-27.5 nmol/L 范围内的睾酮值，两种技术给出的结果大致相同，接近正常范围。
最古老样本中的高平均睾酮水平表明，在过去的三十年中，睾酮水平呈下降趋势，因为任何在储存过程中的降解都可能得出相反的结果。对于存档血清样本，电化学发光免疫分析法和液相色谱串联质谱法均可用于测量预期参考范围内的睾酮。
