Silva Juneo F, Vidigal Paula N, Galvão Daniele D, Boeloni Jankerle N, Nunes Philipe Pimenta, Ocarino Natália M, Nascimento Ernane F, Serakides Rogéria
Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais. 31270-901 Belo Horizonte, MG, Brazil.
Reprod Fertil Dev. 2012;24(7):923-31. doi: 10.1071/RD11219.
The objective of this study was to evaluate fetal weight, histomorphometric changes and proliferative activity, apoptosis and angiogenesis of the placenta in rats with hypothyroidism. Thirty-six adult female rats were divided into two groups with 18 animals each: control and hypothyroidism. Hypothyroidism was induced by daily administration of propylthiouracil (1 mg/animal). The administration began five days before becoming pregnant and the animals were sacrificed at 14 or 19 days of gestation. The control group received a placebo. The number and weight of fetuses and the rate of fetal death was determined, as well as the morphometric characteristics, the immunohistochemical expression of cell division control protein 47 (CDC)-47 and vascular endothelial growth factor (VEGF) and the number of apoptotic cells in the placental disk. The data were analysed by Mann-Whitney U test. Hypothyroidism reduced the weight of fetuses and of the uterus and placenta (P<0.05), altered the thickness of the placental labyrinth and spongiotrophoblast (P<0.05), increased the population of glycogen cells in the spongiotrophoblast (P<0.05), interfered with the vascular development of the placental labyrinth and decreased VEGF expression (P<0.05), reduced the expression of CDC-47 and cellularity and increased the apoptotic rate in the placental disk (P<0.05). We conclude that hypothyroidism affects fetal weight by altering the proliferative activity, apoptosis and vascularisation of the placenta.
本研究的目的是评估甲状腺功能减退大鼠的胎儿体重、组织形态计量学变化以及胎盘的增殖活性、细胞凋亡和血管生成情况。36只成年雌性大鼠被分为两组，每组18只：对照组和甲状腺功能减退组。通过每日给予丙硫氧嘧啶（1毫克/只动物）诱导甲状腺功能减退。给药在怀孕前5天开始，动物在妊娠14天或19天时处死。对照组接受安慰剂。测定胎儿的数量和体重以及胎儿死亡率，以及胎盘盘的形态计量学特征、细胞分裂控制蛋白47（CDC）-47和血管内皮生长因子（VEGF）的免疫组化表达以及凋亡细胞数量。数据采用曼-惠特尼U检验进行分析。甲状腺功能减退降低了胎儿、子宫和胎盘的重量（P<0.05），改变了胎盘迷路和海绵滋养层的厚度（P<0.05），增加了海绵滋养层中糖原细胞的数量（P<0.05），干扰了胎盘迷路的血管发育并降低了VEGF表达（P<0.05），降低了CDC-47的表达和细胞密度，并增加了胎盘盘中的凋亡率（P<0.05）。我们得出结论，甲状腺功能减退通过改变胎盘的增殖活性、细胞凋亡和血管化来影响胎儿体重。
