Espindola-Lozano Marlen, Méndez-Tepepa Maribel, Castillo-Romano Marlenne, Rojas-Juárez Rubicela, Nicolás-Toledo Leticia, Rodríguez-Antolín Jorge, Castelán Francisco, Cuevas-Romero Estela
Ph.D. Program in Biological Sciences, Autonomous University of Tlaxcala, 90070 Tlaxcala, Mexico.
Master Program in Biological Sciences, Autonomous University of Tlaxcala, 90070 Tlaxcala, Mexico.
Metabolites. 2025 Jan 30;15(2):82. doi: 10.3390/metabo15020082.
: To determine the impact of hypothyroidism on uterine glycogen accumulation during pregnancy. : Non-pregnant and pregnant (days 5, 10, and 20) rabbits were grouped into control and methimazole (MMI) groups. In rabbits, serum concentrations of thyroxine (T4), triiodothyronine, glucose, insulin, progesterone, and estradiol were quantified. In uterine inter- and implantation sites, the glycogen content and expression of lactate dehydrogenase (LDH), GLUT4, and aromatase were quantified via Western blot. Fetuses' characteristics at 20 days of pregnancy were analyzed. Two-way ANOVA was used to compare variables between groups. : Pregnancy reduced T4 concentrations but not T3. In virgin groups, MMI treatment significantly reduced the concentrations of T4 and T3 and increased the expression of GLUT4 and aromatase in the uterus compared to the control group. In pregnant groups, T4, T3, glucose, insulin, progesterone, and estradiol levels were similar between control and MMI-treated rabbits. Compared to controls, MMI treatment in pregnant rabbits (a) reduced GLUT4 expression on inter-implantation sites on day 5; (b) increased glycogen content on implantation sites but reduced GLUT4 expression on inter-and implantation sites on day 10; (c) increased glycogen content and LDH and aromatase expression but reduced GLUT4 on inter-implantation sites; and (d) increased glycogen content and the expression of LDH, GLUT4, and aromatase on day 20 on implantation sites. Moreover, the fetus characteristics were similar between groups. : MMI-induced hypothyroidism is associated with changes in the uterine content of glycogen and the expression of LDH, GLUT4, and aromatase during pregnancy.
确定甲状腺功能减退对孕期子宫糖原积累的影响。
将未怀孕和怀孕（第5、10和20天）的兔子分为对照组和甲巯咪唑（MMI）组。对兔子的血清甲状腺素（T4）、三碘甲状腺原氨酸、葡萄糖、胰岛素、孕酮和雌二醇浓度进行定量分析。通过蛋白质免疫印迹法对子宫间质和着床部位的糖原含量以及乳酸脱氢酶（LDH）、葡萄糖转运蛋白4（GLUT4）和芳香化酶的表达进行定量分析。分析怀孕20天时胎儿的特征。采用双向方差分析比较组间变量。
怀孕使T4浓度降低，但T3浓度未降低。在未孕组中，与对照组相比，MMI治疗显著降低了T4和T3的浓度，并增加了子宫中GLUT4和芳香化酶的表达。在怀孕组中，对照组和接受MMI治疗的兔子之间的T4、T3、葡萄糖、胰岛素、孕酮和雌二醇水平相似。与对照组相比，怀孕兔子接受MMI治疗后：（a）第5天在着床间部位GLUT4表达降低；（b）第10天着床部位糖原含量增加，但着床间和着床部位GLUT4表达降低；（c）着床间部位糖原含量、LDH和芳香化酶表达增加，但GLUT4表达降低；（d）第20天着床部位糖原含量以及LDH、GLUT4和芳香化酶的表达增加。此外，各组间胎儿特征相似。
MMI诱导的甲状腺功能减退与孕期子宫糖原含量以及LDH、GLUT4和芳香化酶表达的变化有关。
