开发南美原生树种 Anadenanthera colubrina var. cebil（豆科）的微卫星标记。

Development of microsatellite markers for Anadenanthera colubrina var. cebil (Fabaceae), a native tree from South America.

机构信息

Departamento de Genética, Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Posadas 3300 Misiones, Argentina.

出版信息

Am J Bot. 2012 Sep;99(9):e372-4. doi: 10.3732/ajb.1200078. Epub 2012 Aug 30.

DOI:10.3732/ajb.1200078

PMID:22935360

Abstract

PREMISE OF THE STUDY

Microsatellite primers were developed in the native legume tree Anadenanthera colubrina var. cebil to study the genetic diversity and genetic structure in natural populations in Argentina.

METHODS AND RESULTS

Nine microsatellite markers were identified using a genomic library enriched for tandemly repeated motifs, eight of which markers were polymorphic. The polymorphism of these markers was assessed by investigating 20 individuals for fragment polymorphism; three to 13 alleles were observed for each locus. Observed and expected heterozygosities ranged from 0.300 to 1.000 and from 0.463 to 0.900, respectively.

CONCLUSIONS

These results confirm that these primers will be useful for investigating the genetic diversity and genetic structure of natural populations of A. colubrina var. cebil in future studies.

摘要

研究前提

从原产于豆科植物 Anadenanthera colubrina var. cebil 的植物中开发微卫星引物，以研究阿根廷自然种群的遗传多样性和遗传结构。

方法和结果

使用串联重复基序富集的基因组文库鉴定了 9 个微卫星标记，其中 8 个标记为多态性。通过对 20 个个体的片段多态性进行评估，评估了这些标记的多态性；每个位点观察到 3 到 13 个等位基因。每个位点的观察到的和预期的杂合度分别在 0.300 到 1.000 之间和 0.463 到 0.900 之间。