使用结构域靶向 SELEX 开发和鉴定趋化因子 fractalkine 的适体结合配体。

Development and characterization of an aptamer binding ligand of fractalkine using domain targeted SELEX.

机构信息

Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455, USA.

出版信息

Chem Commun (Camb). 2012 Oct 14;48(80):10043-5. doi: 10.1039/c2cc34217k.

Abstract

Fractalkine (FKN) is a unique cell surface protein with potential as a therapeutic target because of its role in inflammatory diseases and cancer. We developed an aptamer, named FKN-S2, with a dissociation constant of 3.4 ± 0.7 nM that is specific to the chemokine domain of fractalkine.

摘要

趋化因子（FKN）是一种独特的细胞表面蛋白，由于其在炎症性疾病和癌症中的作用，具有成为治疗靶点的潜力。我们开发了一种适体，命名为 FKN-S2，其解离常数为 3.4 ± 0.7 nM，对趋化因子结构域的趋化因子具有特异性。