Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Aesthetic Plast Surg. 2012 Oct;36(5):1254-9. doi: 10.1007/s00266-012-9957-3. Epub 2012 Aug 31.
Plasma has been widely studied and used in many different situations to speed up healing with better tissue adherence and hemostasis. Research projects are now attempting to isolate platelet-rich plasma (PRP) and platelet-poor plasma (PPP), making better use of their properties, particularly during operations and for wounds that are slow to heal. In view of the wide diversity of industrial machines and extraction protocols, together with the variety of industrially produced biologic glues, this article suggests an option for obtaining PRP, PPP, and human thrombin for autologous use.
A way of obtaining PRP, PPP, and thrombin is reproduced through a protocol defined and established by the authors. Autologous thrombin and plasma were obtained through the collection and successive centrifugation of ten whole blood samples, until the desired hemocomponents were isolated, followed by quantitative and qualitative analyses of the elements obtained.
The mean platelet concentration obtained was 6.03 × 10(8) platelets/ml, with a mean thrombin concentration of 33.54 nM, both values compatible with reports in the literature when different protocols are applied.
The protocol described is a good option for the preparation and application of PRP, PPP, and autologous thrombin, particularly as they can be obtained simultaneously, eliminating the possibilities of viral contamination and allergic reactions. Moreover, the cost of this procedure is low, it is easy to perform, and replicable.
This journal requires that authors assign a level of evidence to each article.
等离子体在许多不同的情况下被广泛研究和使用，以加速愈合，具有更好的组织黏附性和止血效果。研究项目现在正试图分离富含血小板的血浆（PRP）和血小板贫血浆（PPP），以更好地利用它们的特性，特别是在手术中和愈合缓慢的伤口中。鉴于工业机器和提取方案的多样性，以及工业生产的生物胶的多样性，本文提出了一种获得 PRP、PPP 和人凝血酶的方法，用于自体使用。
通过作者定义和建立的方案，再现了获得 PRP、PPP 和凝血酶的方法。通过收集和连续离心十个全血样本，获得自体凝血酶和血浆，直到分离出所需的血液成分，然后对获得的元素进行定量和定性分析。
获得的平均血小板浓度为 6.03×10(8) 个/毫升，平均凝血酶浓度为 33.54 nM，这两个值与应用不同方案时文献中的报告值相符。
所描述的方案是制备和应用 PRP、PPP 和自体凝血酶的一个很好的选择，特别是因为它们可以同时获得，消除了病毒污染和过敏反应的可能性。此外，该程序的成本低，易于操作且可复制。
证据等级 IV：本杂志要求作者为每篇文章分配一个证据等级。
