Laboratório de Fisiologia, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil.
Lasers Med Sci. 2013 May;28(3):1007-16. doi: 10.1007/s10103-012-1190-4. Epub 2012 Aug 31.
Following heart failure (HF), immune activation leads to an imbalance between pro-inflammatory and anti-inflammatory cytokines. Low-level laser therapy (LLLT) has been used as an anti-inflammatory treatment in several disease conditions. However, the effect of LLLT on the skeletal muscle of rats with HF remains unclear. The present report aimed to evaluate the influence of LLLT on the inflammatory profile of rats with HF. The left coronary artery was ligated to induce HF and a sham operation was performed in the control groups. Male Wistar rats (n=49) were assigned to one of six groups: placebo sham rats (P-Sham; n=8), LLLT at a dose of 3 J/cm(2) sham rats (3 J/cm(2)-Sham; n=8), LLLT at a dose of 21 J/cm(2) sham rats (21 J/cm(2)-Sham; n=8), placebo HF rats (P-HF; n=9), LLLT at a dose of 3 J/cm(2) HF rats (3 J/cm(2)-HF; n=8), and LLLT at a dose of 21 J/cm(2) HF rats (21 J/cm(2)-HF; n=8). Four weeks after myocardial infarction or sham surgery, rats were subjected to LLLT (InGaAlP 660 nm, spot size 0.035 cm(2), output power 20 mW, power density 0.571 W/cm(2), energy density 3 or 21 J/cm(2), exposure time 5.25 s and 36.75 s) on the right gastrocnemius for 10 consecutive days. LLLT reduced plasma IL-6 levels (61.3 %; P<0.01), TNF-α/IL-10 (61.0 %; P<0.01) and IL-6/IL-10 ratios (77.3 %; P<0.001) and increased IL-10 levels (103 %; P<0.05) in the 21 J/cm(2)-HF group. Moreover, LLLT reduced the TNF-α (20.1 % and 21.3 %; both P<0.05) and IL-6 levels (54.3 % and 37.8 %; P<0.01 and P<0.05, respectively) and the IL-6/IL-10 ratio (59.7 % and 42.2 %; P<0.001 and P<0.05, respectively) and increased IL-10 levels (81.0 % and 85.1 %; both P<0.05) and the IL-10/TNF-α ratio (171.5 % and 119.8 %; P<0.001 and P<0.05, respectively) in the gastrocnemius in the 3 J/cm(2)-HF and 21 J/cm(2)-HF groups. LLLT showed systemic and skeletal muscle anti-inflammatory effects in rats with HF.
心力衰竭（HF）后，免疫激活导致促炎细胞因子和抗炎细胞因子之间失衡。低水平激光疗法（LLLT）已被用作几种疾病状况的抗炎治疗方法。然而，LLLT 对 HF 大鼠骨骼肌的影响尚不清楚。本报告旨在评估 LLLT 对 HF 大鼠炎症谱的影响。结扎左冠状动脉以诱导 HF，并在对照组中进行假手术。雄性 Wistar 大鼠（n=49）分为六组之一：安慰剂假手术组（P-Sham；n=8）、3 J/cm(2)剂量 LLLT 假手术组（3 J/cm(2)-Sham；n=8）、21 J/cm(2)剂量 LLLT 假手术组（21 J/cm(2)-Sham；n=8）、安慰剂 HF 大鼠组（P-HF；n=9）、3 J/cm(2)剂量 LLLT HF 大鼠组（3 J/cm(2)-HF；n=8）和 21 J/cm(2)剂量 LLLT HF 大鼠组（21 J/cm(2)-HF；n=8）。心肌梗塞或假手术后 4 周，大鼠接受 LLLT（InGaAlP 660nm，光斑尺寸 0.035cm(2)，输出功率 20mW，功率密度 0.571W/cm(2)，能量密度 3 或 21J/cm(2)，暴露时间 5.25s 和 36.75s）右比目鱼肌连续 10 天。LLLT 降低了 HF 大鼠的血浆 IL-6 水平（61.3%；P<0.01）、TNF-α/IL-10（61.0%；P<0.01）和 IL-6/IL-10 比值（77.3%；P<0.001），并增加了 IL-10 水平（103%；P<0.05）在 21 J/cm(2)-HF 组。此外，LLLT 降低了 TNF-α（20.1%和 21.3%；均 P<0.05）和 IL-6 水平（54.3%和 37.8%；均 P<0.01 和 P<0.05）以及 IL-6/IL-10 比值（59.7%和 42.2%；均 P<0.001 和 P<0.05），并增加了 IL-10 水平（81.0%和 85.1%；均 P<0.05）和 IL-10/TNF-α 比值（171.5%和 119.8%；均 P<0.001 和 P<0.05）在 3 J/cm(2)-HF 和 21 J/cm(2)-HF 组的比目鱼肌中。LLLT 对 HF 大鼠表现出全身和骨骼肌抗炎作用。
