Ferguson D J, Lee S F, Gordon P A
Department of Laboratory Medicine, University of Alberta Hospitals, Edmonton, Canada.
Am J Hematol. 1990 Jan;33(1):13-7. doi: 10.1002/ajh.2830330104.
Reticulocyte counting using flow cytometry and a membrane-permeable fluorescent dye, thiazole orange, was evaluated as an alternative to the conventional manual method. The flow cytometric method was more precise (mean c.v. of 4.3%) than the imprecise manual technique (mean c.v. of 22.4%). Linearity was highly acceptable (r = 0.99) over the reticulocyte count range of 1.8-30.1%. Flow cytometry allows processing of large numbers of samples with reduction in technologist time, and the improved precision and tenfold increase in the number of cells counted should considerably improve the clinical utility of the reticulocyte count.
使用流式细胞术和一种可透过细胞膜的荧光染料噻唑橙进行网织红细胞计数，作为传统手工方法的替代方法进行了评估。流式细胞术方法比不精确的手工技术更精确（平均变异系数为4.3%，而手工技术的平均变异系数为22.4%）。在1.8 - 30.1%的网织红细胞计数范围内，线性度非常令人满意（r = 0.99）。流式细胞术允许处理大量样本，减少了技术人员的时间，并且提高的精度和计数细胞数量增加了十倍，应该会大大提高网织红细胞计数的临床实用性。
