Bowen D, Bentley N, Hoy T, Cavill I
Department of Haematology, University Hospital of Wales, Cardiff.
J Clin Pathol. 1991 Feb;44(2):130-3. doi: 10.1136/jcp.44.2.130.
Two independent methods for quantitating reticulocyte counts were compared. One used a modified thiazole orange technique and a flow cytometer (Becton Dickinson FACS); the other was a fully automated whole blood analyser (Sysmex R1000). Both methods gave comparable results with a coefficient of variation of less than 5%. Samples measured using the R1000 showed a negligible decrease in the reticulocyte count over five days at room temperature, although there was evidence of continuing intracellular maturation: with thiazole orange there was an apparent increase. A practical reference range of 20-70 x 10(9)/l was established from 89 normal subjects. The close correlation between the two independent estimates indicates the validity of the quantitation of the reticulocyte count and shows that automation allows significant changes within and below the normal range to be detected with a degree of reliability which was not previously possible.
对两种独立的网织红细胞计数定量方法进行了比较。一种方法采用改良噻唑橙技术和流式细胞仪（贝克曼库尔特FACS）；另一种是全自动全血分析仪（Sysmex R1000）。两种方法得出的结果相当，变异系数小于5%。使用R1000测量的样本在室温下放置五天后网织红细胞计数的下降可忽略不计，尽管有证据表明细胞内仍在持续成熟：而使用噻唑橙时则出现明显增加。从89名正常受试者中建立了20 - 70×10⁹/L的实际参考范围。两种独立估计值之间的密切相关性表明了网织红细胞计数定量的有效性，并表明自动化能够以先前无法实现的可靠程度检测正常范围内及以下的显著变化。
