Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Biomaterials. 2012 Nov;33(33):8221-7. doi: 10.1016/j.biomaterials.2012.08.035. Epub 2012 Aug 28.
Development of living cell-based devices holds great promise in many biomedical and industrial applications. To increase our understanding of the process, we investigated the biological and electrochemical properties of a redox phospholipid polymer hydrogel containing an electron-generating bacteria (Shewanella oneidensis MR-1). A water-soluble and amphiphilic phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid-co-vinylferrocene) (PMBVF), was our choice for incorporation into a hydrogel matrix that promotes encapsulation of bacteria and acts as an electron transfer mediator. This hydrogel formed spontaneously and encapsulated Shewanella in three-dimensional structures. Visual analysis showed that the encapsulated Shewanella maintained viability and metabolic activity even after long-term storage. Cyclic voltammetry measurement indicated that the PMBVF/poly(vinyl alcohol) (PMBVF/PVA) hydrogel had stable and high electron transfer efficiency. Amperometric measurement showed that the hydrogel could maintain the electron transfer efficiency even when Shewanella was encapsulated. Thus, the PMBVF/PVA hydrogel not only provides a mild environment for long-term bacterial survival but also maintains electron transfer efficiency from the bacteria to the electrode. We conclude that hydrogel/bacteria hybrid biomaterials, such as PMBVF/PVA/Shewanella, may find application in the fabrication of living cell-based devices.
基于活细胞的器件在许多生物医学和工业应用中具有巨大的应用前景。为了增进我们对这一过程的理解，我们研究了含有电子产生细菌（希瓦氏菌属 oneidensis MR-1）的氧化还原磷脂聚合物水凝胶的生物和电化学性质。我们选择了一种水溶性的两亲性磷脂聚合物，聚（2-甲基丙烯酰氧基乙基磷酸胆碱-co-n-丁基甲基丙烯酸酯-co-p-乙烯基苯硼酸-co-乙烯基二茂铁）（PMBVF），将其掺入水凝胶基质中，以促进细菌的封装并充当电子转移介质。这种水凝胶可以自发形成，并将希瓦氏菌封装在三维结构中。直观分析表明，即使在长期储存后，封装的希瓦氏菌仍保持活力和代谢活性。循环伏安法测量表明，PMBVF/聚乙烯醇（PMBVF/PVA）水凝胶具有稳定且高效的电子转移效率。安培测量表明，即使封装了希瓦氏菌，水凝胶仍能保持电子转移效率。因此，PMBVF/PVA 水凝胶不仅为细菌的长期生存提供了温和的环境，而且还保持了细菌到电极的电子转移效率。我们得出结论，水凝胶/细菌杂化生物材料，例如 PMBVF/PVA/Shewanella，可能会在基于活细胞的器件的制造中得到应用。
