Analytical division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi-221005, India.
Biosens Bioelectron. 2013 Jan 15;39(1):236-43. doi: 10.1016/j.bios.2012.07.080. Epub 2012 Aug 17.
This study describes the synthesis of a new class of substrate-selective molecularly imprinted polymer. This involved tetraethylene glycol 3-morpholin propionate acrylate (functional monomer) and bovine serum albumin (template) for polymerization in aqueous condition, using "surface grafting-from" approach directly on a vinyl exposed multiwalled carbon nanotubes-ceramic electrode. The analyte recapture at pH 6.8 in aqueous environment simultaneously involved hydrophobically driven hydrogen bonds and ionic interactions between negatively charged bovine serum albumin and positively charged imprinted nanofilm. The selectively encapsulated bovine serum albumin first gets reduced at -0.9V and then oxidized within the cavity, without getting stripped off, to respond a differential pulse voltammetry signal. The limit of detection [0.42ng mL(-1) (3σ, RSD≤1.02%)] obtained was free from any cross-reactivity and matrix complications in aqueous, pharmaceutical, serum, and liquid milk samples. The proposed sensor can be used as a practical sensor for ultra-trace analysis of bovine serum albumin in clinical settings.
本研究描述了一类新型的底物选择性分子印迹聚合物的合成。该方法涉及四乙二醇 3-吗啉丙基丙烯酸酯（功能单体）和牛血清白蛋白（模板），在水相条件下，采用“表面接枝-从”方法，直接在乙烯暴露的多壁碳纳米管-陶瓷电极上聚合。在 pH 6.8 的水溶液环境中，分析物的再捕获同时涉及疏水力驱动的氢键和带负电荷的牛血清白蛋白与带正电荷的印迹纳米膜之间的离子相互作用。被选择性包封的牛血清白蛋白首先在-0.9V 下还原，然后在空腔内氧化，而不会被剥离，以响应差分脉冲伏安法信号。在水相、药物、血清和液态奶样品中，获得的检测限[0.42ng mL(-1)（3σ，RSD≤1.02%）]没有任何交叉反应和基质干扰。该传感器可作为临床超痕量分析牛血清白蛋白的实用传感器。
