Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China; Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
Biosens Bioelectron. 2015 Dec 15;74:792-8. doi: 10.1016/j.bios.2015.07.046. Epub 2015 Jul 21.
A molecularly imprinted polymer film was in situ polymerized on a carboxyl functionalized multi-walled carbon nanotubes modified glassy carbon electrode surface under room temperature. This technique provides a promising imprinting approach for protein in an aqueous solution using 3-(3-aminopropyl)-1-vinylimidazolium tetrafluoroborate ionic liquid as functional monomer, N, N'-methylenebisacrylamide as crossing linker, ammonium persulfate and N,N,N',N'-tetramethylethylenediamine as initiator, and bovine serum albumin (BSA) as template. The molecularly imprinted polymerized ionic liquid film shows enhanced accessibility, high specificity and sensitivity towards BSA. Electrochemical sensing performance of the imprinted sensor was thoroughly investigated using K3Fe[CN]6/K4Fe[CN]6 as electroactive probes. Under optimal conditions, the current difference before and after specific recognition of BSA was found linearly related to its concentration in the range from 1.50×10(-9) to 1.50×10(-6) mol L(-1). The detection limit was calculated to be 3.91×10(-10) mol L(-1) (S/N=3). The practical application of the imprinted sensor was demonstrated by determining BSA in liquid milk samples.
在室温下，将分子印迹聚合物膜原位聚合在羧基功能化多壁碳纳米管修饰的玻碳电极表面。该技术使用 3-(3-氨丙基)-1-乙烯基咪唑四氟硼酸盐离子液体作为功能单体、N,N'-亚甲基双丙烯酰胺作为交联剂、过硫酸铵和 N,N,N',N'-四甲基乙二胺作为引发剂，以牛血清白蛋白（BSA）为模板，在水溶液中对蛋白质提供了一种有前途的印迹方法。分子印迹聚合离子液体膜对 BSA 具有增强的可及性、高特异性和高灵敏度。使用 K3Fe[CN]6/K4Fe[CN]6 作为电活性探针，对印迹传感器的电化学传感性能进行了深入研究。在最佳条件下，BSA 特异性识别前后的电流差与浓度在 1.50×10(-9)至 1.50×10(-6) mol L(-1)范围内呈线性关系。检测限计算为 3.91×10(-10) mol L(-1)（S/N=3）。通过测定液态奶样品中的 BSA，验证了印迹传感器的实际应用。
