Department of Chemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India.
Phytomedicine. 2012 Oct 15;19(13):1228-33. doi: 10.1016/j.phymed.2012.07.015. Epub 2012 Aug 29.
To evaluate the in vitro anti-proliferative and radical scavenging properties of the essential oil and its fractions and to determine the chemo-type of P. wallichiana essential oil.
Pinus wallichiana oil was extracted by hydro-distillation and fractionated by silica gel column chromatography method. The oil and its fractions were analyzed by Gas chromatography, Gas chromatography-mass spectrometry and (13)C NMR. Different concentrations of oil 12.5, 25, 50 and 100μg/ml and single concentration 50μg/ml of its fractions B(1), B(2), A(2), G(2), Uk(13) and I(2) were evaluated for its anti-proliferative activity by in vitro {3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide} assay against human monocyte, lung carcinoma, liver adenocarcinoma, prostate and ovarian carcinoma, while as the radical scavenging activity was evaluated by different in vitro DPPH assays.
The analyses indicated the presence of 17 constituents with β-pinene (46.8%) and α-pinene (25.2%) as major constituents. The oil and its fractions showed significant anti-proliferative activity. The radical scavenging activity also showed good results.
The oil could be used as a drug to control the diseases like cancer, cirrhosis and arteriosclerosis, caused by reactive oxygen species.
评估挥发油及其馏分的体外抗增殖和自由基清除特性，并确定松节油的化学型。
采用水蒸馏法从松节油中提取油，并用硅胶柱色谱法对其进行分离。通过气相色谱、气相色谱-质谱联用和（13）C NMR 对油及其馏分进行分析。用体外{3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐}法评估油及其馏分 B(1)、B(2)、A(2)、G(2)、Uk(13)和 I(2)在 12.5、25、50 和 100μg/ml 等不同浓度和 50μg/ml 单浓度下对人单核细胞、肺癌、肝癌、前列腺癌和卵巢癌细胞的抗增殖活性，而自由基清除活性则通过不同的体外 DPPH 法进行评估。
分析表明，该油含有 17 种成分，主要成分为β-蒎烯（46.8%）和α-蒎烯（25.2%）。油及其馏分表现出显著的抗增殖活性。自由基清除活性也表现出良好的效果。
该油可作为一种药物，用于控制由活性氧引起的癌症、肝硬化和动脉硬化等疾病。
