Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
J Formos Med Assoc. 2012 Aug;111(8):427-30. doi: 10.1016/j.jfma.2011.06.018. Epub 2012 Apr 30.
BACKGROUND/PURPOSE: Rapid diagnosis of influenza was important in the global pandemic influenza A/H1N1 outbreak. The QuickVue Influenza A+B test is one of the most common tests for rapid diagnosis of influenza. We evaluated the sensitivity and specificity of the test in children.
We collected data from 970 patients with influenza-like illness who received rapid influenza antigen tests using the QuickVue Influenza A+B test as well as viral isolation. We compared the results with that of viral isolation and reverse-transcriptase polymerase chain reaction (RT-PCR) assays.
Based on viral culture, the QuickVue Influenza A+B test had an overall sensitivity of 0.82 (419/513) and specificity of 0.99 (70/71), with a positive predictive value (PPV) of 0.74 (419/563) and a negative predictive value (NPV) of 0.77 (313/407). The sensitivity of QuickVue was significantly higher in specimens with high viral loads. If the viral loads were less than 10(6), the sensitivity of QuickVue was 0.62, while the sensitivity of QuickVue was 0.88 if the viral loads were higher than 10(6) (p<0.001). Logistic regression analysis showed that higher viral loads correlated with positive QuickVue results (p<0.001). On the first day of fever, the sensitivity of QuickVue was only 0.67; on the second day, the sensitivity was 0.86; on the third day, the sensitivity was 0.98, and on the fourth day, the sensitivity was 0.90. The sensitivity is significantly higher on days 2-3 in comparison with the first day of fever (p<0.05). We calculated the correlation of viral load and fever days, and the result showed higher mean viral load on the second and third days of fever. Age did not affect the sensitivity.
In children, the sensitivity of QuickVue Influenza A+B test was 0.82. In addition, the sensitivity was significantly elevated in the higher viral load group and on the third day of fever.
背景/目的：流感的快速诊断在全球甲型 H1N1 流感大流行中非常重要。QuickVue 流感 A+B 检测是用于快速诊断流感的最常见检测方法之一。我们评估了该检测方法在儿童中的敏感性和特异性。
我们收集了 970 例流感样疾病患者的数据，这些患者使用 QuickVue 流感 A+B 检测进行了快速流感抗原检测，同时进行了病毒分离。我们将结果与病毒分离和逆转录聚合酶链反应（RT-PCR）检测进行了比较。
根据病毒培养，QuickVue 流感 A+B 检测的总敏感性为 0.82（419/513），特异性为 0.99（70/71），阳性预测值（PPV）为 0.74（419/563），阴性预测值（NPV）为 0.77（313/407）。在病毒载量较高的标本中，QuickVue 的敏感性明显更高。如果病毒载量低于 10（6），QuickVue 的敏感性为 0.62，而如果病毒载量高于 10（6），QuickVue 的敏感性为 0.88（p<0.001）。逻辑回归分析表明，较高的病毒载量与 QuickVue 阳性结果相关（p<0.001）。在发热的第一天，QuickVue 的敏感性仅为 0.67；在第二天，敏感性为 0.86；在第三天，敏感性为 0.98，在第四天，敏感性为 0.90。与发热的第一天相比，第二天和第三天的敏感性明显更高（p<0.05）。我们计算了病毒载量和发热天数的相关性，结果显示发热的第二天和第三天病毒载量较高。年龄不影响敏感性。
在儿童中，QuickVue 流感 A+B 检测的敏感性为 0.82。此外，在病毒载量较高的组和发热的第三天，敏感性明显升高。
