Goldstone Jared V, Stegeman John J
Biology Department, Redfield 3-42 MS#32, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA.
Mar Environ Res. 2008 Jul;66(1):19-20. doi: 10.1016/j.marenvres.2008.02.011. Epub 2008 Feb 26.
The cytochrome P450 1 (CYP1) family has expanded with the addition of the CYP1B and CYP1C subfamilies. We recently identified a new CYP1 subfamily in zebrafish, CYP1D, with a single gene, CYP1D1. Here we examined sequences found in other fish genomes, i.e., stickleback (Gasterosteus aculeatus) and medaka (Oryzias latipes), for similarities among fish CYP1D1 genes. The full-length deduced amino acid sequences for CYP1D1 in these two species averaged about 43% identity to the CYP1As, but nearly 50% when sequence alignment ambiguities were masked. CYP1D1 has seven exons, similar in size and position to the exons in CYP1D1 and CYP1A in zebrafish. However, the intronic distances were substantially smaller in the medaka and stickleback. There also were differing numbers of putative xenobiotic response elements in the CYP1D1 of the various species. Whether the stickleback or medaka genes are inducible by aryl hydrocarbon receptor (AHR) agonists is yet to be determined.
细胞色素P450 1（CYP1）家族因新增CYP1B和CYP1C亚家族而得到扩展。我们最近在斑马鱼中鉴定出一个新的CYP1亚家族，即CYP1D，它只有一个基因CYP1D1。在此，我们研究了在其他鱼类基因组中发现的序列，即棘鱼（刺鱼属）和青鳉的序列，以探寻鱼类CYP1D1基因之间的相似性。这两个物种中CYP1D1推导的全长氨基酸序列与CYP1A的平均一致性约为43%，但在掩盖序列比对中的模糊区域后，一致性接近50%。CYP1D1有七个外显子，其大小和位置与斑马鱼CYP1D1和CYP1A中的外显子相似。然而，青鳉和棘鱼中内含子的长度要短得多。不同物种的CYP1D1中推定的外源性物质反应元件数量也有所不同。棘鱼或青鳉的基因是否能被芳烃受体（AHR）激动剂诱导还有待确定。
