Department of Environmental Science, Zhejiang University, Hangzhou 310058, China.
Bioresour Technol. 2012 Nov;123:42-8. doi: 10.1016/j.biortech.2012.07.059. Epub 2012 Aug 2.
In order to better understand how surfactants affect biodegradation of hydrophobic organic compounds (HOCs), Tween 80 and sodium dodecyl benzene sulfonate (SDBS), were selected to investigate effects on cell surface hydrophobicity (CSH), electron transport system (ETS) activities and phenanthrene biodegradation by Citrobacter sp. SA01. Tween 80 and SDBS increased CSH by 19.8-25.2%, ETS activities by 352.1-376.0μmol/gmin, catechol 1,2-dioxygenase (C12) activities by 50.8-52.7U/L, and phenanthrene biodegradation by 8.9-17.2% separately in the presence of 50mg/L of surfactants as compared to in their absence. Lipopolysaccharide (LPS) release was 334.7μg/mg in the presence of both surfactants whereas in their absence only 8.6-44.4μg/mg of LPS was released. Thus, enhanced LPS release probably increased ETS and C12 activities as well as phenanthrene biodegradation by increasing CSH. The results demonstrate that surfactant-enhanced CSH provides a simple, yet effective strategy for field applications of surfactant-enhanced bioremediation of HOCs.
为了更好地了解表面活性剂如何影响疏水性有机化合物（HOCs）的生物降解，选择吐温 80 和十二烷基苯磺酸钠（SDBS）来研究它们对细胞表面疏水性（CSH）、电子传递系统（ETS）活性和柠檬酸杆菌 SA01 对菲的生物降解的影响。与不存在表面活性剂相比，在存在 50mg/L 表面活性剂的情况下，吐温 80 和 SDBS 分别将 CSH 增加了 19.8-25.2%、ETS 活性增加了 352.1-376.0μmol/gmin、儿茶酚 1,2-双加氧酶（C12）活性增加了 50.8-52.7U/L，菲的生物降解增加了 8.9-17.2%。在存在这两种表面活性剂的情况下，脂多糖（LPS）的释放量为 334.7μg/mg，而在不存在表面活性剂的情况下，LPS 的释放量仅为 8.6-44.4μg/mg。因此，增强的 LPS 释放可能通过增加 CSH 来增加 ETS 和 C12 活性以及菲的生物降解。结果表明，表面活性剂增强的 CSH 为 HOCs 的表面活性剂强化生物修复的现场应用提供了一种简单而有效的策略。
