Department of Environmental Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process Control, Hangzhou 310058, China; Zhejiang Yuying College, Hangzhou 310018, China.
Department of Environmental Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process Control, Hangzhou 310058, China.
Chemosphere. 2014 Jul;107:58-64. doi: 10.1016/j.chemosphere.2014.03.016. Epub 2014 Apr 12.
The effects of the surfactants, Tween 80 and sodium dodecyl benzene sulfonate (SDBS) on a membrane's fatty acid composition and the transmembrane transport of phenanthrene were investigated. The results indicated that both surfactants could modify the composition of fatty acids of Citrobacter sp. Strain SA01 cells, 50 mg L(-1) of both surfactants changed the composition of the fatty acids the most, increasing the amount of unsaturated fatty acids. The comparison of fatty acid profiles with diphenylhexatriene fluorescence anisotropy, a probe for plasma membrane fluidity, suggested that an increased amount of unsaturated fatty acids corresponded to greater membrane fluidity. In addition, increased unsaturated fatty acids promoted phenanthrene to partition from the extracellular matrix to cell debris, which increased reverse partitioning from the cell debris to the cytochylema. The results of this study were expected in that the addition of a surfactant is a simple and effective method for accelerating the rate-limiting step of transmembrane transport of hydrophobic organic compounds (HOCs) in bioremediation.
研究了表面活性剂吐温 80 和十二烷基苯磺酸钠（SDBS）对细胞膜脂肪酸组成和菲跨膜转运的影响。结果表明，两种表面活性剂均可改变Citrobacter sp. Strain SA01 细胞的脂肪酸组成，浓度为 50mg/L 的两种表面活性剂对脂肪酸组成的改变最大，增加了不饱和脂肪酸的含量。与二苯基十六碳三烯荧光各向异性（一种用于测量质膜流动性的探针）的脂肪酸图谱比较表明，不饱和脂肪酸含量的增加对应于更大的膜流动性。此外，增加的不饱和脂肪酸促进菲从细胞外基质向细胞碎片分配，这增加了从细胞碎片向细胞质的反向分配。该研究的结果是预期的，因为添加表面活性剂是加速生物修复中疏水性有机化合物（HOCs）跨膜转运限速步骤的简单有效方法。
