Sino-Forest Applied Research Centre for Pearl River Delta Environment and Department of Biology, Hong Kong Baptist University, Hong Kong SAR, PR China.
Bioresour Technol. 2011 Mar;102(5):3999-4007. doi: 10.1016/j.biortech.2010.11.088. Epub 2010 Nov 25.
The effects of rhamnolipids produced by Pseudomonas aeruginosa ATCC9027 on the cell surface hydrophobicity (CSH) and the biodegradation of phenanthrene by two thermophilic bacteria, Bacillus subtilis BUM and P. aeruginosa P-CG3, and mixed inoculation of these two strains were investigated. Rhamnolipids significantly reduced the CSH of the hydrophobic BUM and resulted in a noticeable lag period in the biodegradation. However, they significantly increased the CSH and enhanced the biodegradation for the hydrophilic P-CG3. In the absence of rhamnolipids, a mixed inoculation of BUM and P-CG3 removed 82.2% of phenanthrene within 30 days and the major contributor of the biodegradation was BUM (rapid degrader) while the growth of P-CG3 (slow degrader) was suppressed. Addition of rhamnolipids promoted the surfactant-mediated-uptake of phenanthrene by P-CG3 but inhibited the uptake through direct contact by BUM. This resulted in the domination of P-CG3 during the initial stage of biodegradation and enhanced the biodegradation to 92.7%.
研究了铜绿假单胞菌 ATCC9027 产生的鼠李糖脂对两种嗜热细菌枯草芽孢杆菌 BUM 和铜绿假单胞菌 P-CG3 的细胞表面疏水性（CSH）和菲生物降解的影响，以及这两种菌株的混合接种。鼠李糖脂显著降低了疏水性 BUM 的 CSH，并导致生物降解出现明显的滞后期。然而，它们显著增加了亲水性 P-CG3 的 CSH 并增强了其生物降解能力。在没有鼠李糖脂的情况下，BUM 和 P-CG3 的混合接种在 30 天内去除了 82.2%的菲，生物降解的主要贡献者是 BUM（快速降解菌），而 P-CG3（缓慢降解菌）的生长受到抑制。鼠李糖脂的添加促进了 P-CG3 通过表面活性剂介导的菲摄取，但抑制了 BUM 通过直接接触的摄取。这导致在生物降解的初始阶段 P-CG3 的主导地位，并将生物降解提高到 92.7%。
