Department of Pathology, University of Vermont College of Medicine, 89 Beaumont Avenue, Burlington, VT 05405, USA.
Histochem Cell Biol. 2012 Nov;138(5):815-20. doi: 10.1007/s00418-012-1024-2. Epub 2012 Sep 1.
Cell adhesion molecule 1 (CADM1) is a member of the immunoglobulin cell adhesion molecule family. Recently, we identified CADM1 to be a novel risk factor for venous thrombosis in a large, protein C deficient, thrombophilic family and showed, for the first time, the expression of CADM1 in endothelial cells (Hasstedt et al. in Blood 114:3084-3091, 2009). To further investigate its role in venous thrombosis, as well as other vasculopathies, we undertook a systematic confocal microscopic investigation for the presence of CADM1 in the vasculature of 28 different human tissues. Paraffin embedded tissue sections were dual immunostained with an antibody against CADM1, together with an antibody against either von Willebrand factor (to identify endothelial cells), or α-smooth muscle actin (to identify smooth muscle cells). The results showed that CADM1 was ubiquitously present in endothelial cells and smooth muscle cells in the vasculature from all 28 tissues, though its representation in the various classes of vessels was tissue dependent.
细胞黏附分子 1（CADM1）是免疫球蛋白细胞黏附分子家族的一员。最近，我们在一个大型蛋白 C 缺乏的血栓形成家族中发现 CADM1 是静脉血栓形成的一个新的危险因素，并首次显示 CADM1 在血管内皮细胞中的表达（Hasstedt 等人，Blood 114:3084-3091, 2009）。为了进一步研究 CADM1 在静脉血栓形成以及其他血管病变中的作用，我们对 28 种不同的人体组织中的血管中 CADM1 的存在进行了系统的共聚焦显微镜研究。用针对 CADM1 的抗体与针对 von Willebrand 因子（识别内皮细胞）或α-平滑肌肌动蛋白（识别平滑肌细胞）的抗体对石蜡包埋的组织切片进行双重免疫染色。结果表明，CADM1 普遍存在于来自所有 28 种组织的血管内皮细胞和血管平滑肌细胞中，但在各种血管类型中的表达则依赖于组织。
