Yuan Jiayin, Kihara Takako, Kimura Neinei, Yamasaki Takashi, Yoshida Makoto, Isozaki Koji, Ito Akihiko, Hirota Seiichi
Department of Surgical Pathology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan.
Department of Pathology, Faculty of Medicine, Kindai University, Osaka-Sayama, Osaka 589-8511, Japan.
Oncol Lett. 2022 Mar;23(3):86. doi: 10.3892/ol.2022.13206. Epub 2022 Jan 19.
Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the human gastrointestinal tract. Small intestinal GISTs appear to be associated with poorer prognosis and higher metastasis rate than gastric GISTs of the same size and mitotic index. Recently, we reported that cell adhesion molecule 1 () is expressed specifically in most small intestinal GISTs, but not in most gastric GISTs, suggesting that this difference in expression between gastric GISTs and small intestinal GISTs might influence the difference in clinical behavior between them. The aim of the present study was to examine whether high expression affected proliferation, migration, invasion, adhesion to endothelial cells and transendothelial migration of cultured GIST cells by comparing original GIST-T1 cells with very low expression with GIST-T1 cells with high expression induced by cDNA transfection (GIST-T1-CAD cells). GIST-T1-CAD cells had reduced ability to proliferate, migrate and invade compared with the original GIST-T1 cells, but showed significantly higher ability to adhere to human umbilical vein endothelial cells and migrate through endothelial cell monolayers. Thus, CADM1 may contribute to higher metastasis rates in small intestinal GISTs facilitating tumor cell adhesion to vascular endothelial cell and transendothelial migration of tumor cells. CADM1 might serve as a potential target for inhibition of metastasis in small intestinal GISTs.
胃肠道间质瘤（GIST）是人类胃肠道最常见的间充质肿瘤。与相同大小和有丝分裂指数的胃GIST相比，小肠GIST似乎预后较差且转移率较高。最近，我们报道细胞粘附分子1（）在大多数小肠GIST中特异性表达，但在大多数胃GIST中不表达，这表明胃GIST和小肠GIST之间这种表达差异可能影响它们之间临床行为的差异。本研究的目的是通过比较低表达的原始GIST-T1细胞与经cDNA转染诱导的高表达GIST-T1细胞（GIST-T1-CAD细胞），研究高表达是否影响培养的GIST细胞的增殖、迁移、侵袭、与内皮细胞的粘附及跨内皮迁移。与原始GIST-T1细胞相比，GIST-T1-CAD细胞的增殖、迁移和侵袭能力降低，但显示出与人脐静脉内皮细胞粘附及穿过内皮细胞单层迁移的能力显著增强。因此，CADM1可能导致小肠GIST转移率较高，促进肿瘤细胞与血管内皮细胞的粘附及肿瘤细胞的跨内皮迁移。CADM1可能作为抑制小肠GIST转移的潜在靶点。
