State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
Opt Lett. 2012 Sep 1;37(17):3630-2. doi: 10.1364/OL.37.003630.
An auto-focusing method for digital holographic microscopy has been proposed by employing two off-axis illumination beams. When specimens are illuminated by two plane waves in different directions, it is found that the farther the reconstruction plane is from the image plane, the wider the two reconstructed images are separated from each other. Thus, the image plane can be determinated by finding the minimum of the variation between the two reconstructed object waves on both the amplitude and phase distributions. The feasibility of the proposed method is demonstrated by the corresponding simulation and experiment.
提出了一种利用两离轴照明光束实现数字全息显微镜自动聚焦的方法。当用两个不同方向的平面波照明样品时，发现离像面越远，两个重建像之间的分离就越大。因此，可以通过在两个物体波的振幅和相位分布上找到两个重建物波之间变化的最小值来确定像面。通过相应的模拟和实验验证了该方法的可行性。
