ITMO University, Department of Photonics and Optical Information Technologies, Saint-Petersburg, 199034, Russia.
Sci Rep. 2017 Mar 2;7:43840. doi: 10.1038/srep43840.
We propose a new express method of the correlation characterization of the particles suspended in the volume of optically transparent medium. It utilizes inline digital holography technique for obtaining two images of the adjacent layers from the investigated volume with subsequent matching of the cross-correlation function peak-to-basement ratio calculated for these images. After preliminary calibration via numerical simulation, the proposed method allows one to quickly distinguish parameters of the particle distribution and evaluate their concentration. The experimental verification was carried out for the two types of physical suspensions. Our method can be applied in environmental and biological research, which includes analyzing tools in flow cytometry devices, express characterization of particles and biological cells in air and water media, and various technical tasks, e.g. the study of scattering objects or rapid determination of cutting tool conditions in mechanisms.
我们提出了一种新的表达方法，用于关联描述光学透明介质体积中悬浮颗粒的特征。它利用在线数字全息技术从研究体积中获取两个相邻层的图像，然后对这些图像计算的互相关函数峰值与基底比进行匹配。通过数值模拟进行初步校准后，该方法允许快速区分颗粒分布参数并评估其浓度。实验验证是针对两种类型的物理悬浮液进行的。我们的方法可应用于环境和生物学研究，包括在流式细胞仪设备中分析工具，以及在空气和水介质中对颗粒和生物细胞进行快速特征描述，还可应用于各种技术任务，例如散射物体的研究或在机构中快速确定刀具条件。
