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多瘤病毒晚期信使核糖核酸的剪接不在被感染细胞的细胞质中发生。

Splicing of the late mRNAs of polyoma virus does not occur in the cytoplasm of the infected cell.

作者信息

Piper P, Wardale J, Crew F

出版信息

Nature. 1979 Dec 13;282(5740):686-91. doi: 10.1038/282686a0.

DOI:10.1038/282686a0
PMID:229415
Abstract

The three mRNAs that encode the capsid proteins of polyoma virus are produced by the excision of different sequences from continuous transcripts of the L strand of viral DNA. All three of the mRNAs have long half lives, and the larger species are not converted to the smaller ones to any measurable extent within the cytoplasm. Therefore the cytoplasmic proportions of late polyoma mRNAs are predetermined by splicing that is confined to the nucleus of the infected cell and which is complete by the time that mRNA is transported to the cytoplasm.

摘要

编码多瘤病毒衣壳蛋白的三种信使核糖核酸(mRNA)是通过从病毒DNA的L链连续转录本中切除不同序列而产生的。所有这三种mRNA都具有较长的半衰期,并且在细胞质中,较大的mRNA种类不会以任何可测量的程度转化为较小的种类。因此,晚期多瘤病毒mRNA在细胞质中的比例是由仅限于受感染细胞核内的剪接预先确定的,并且在mRNA转运到细胞质时剪接已经完成。

相似文献

1
Splicing of the late mRNAs of polyoma virus does not occur in the cytoplasm of the infected cell.多瘤病毒晚期信使核糖核酸的剪接不在被感染细胞的细胞质中发生。
Nature. 1979 Dec 13;282(5740):686-91. doi: 10.1038/282686a0.
2
Multiple 5' terminal cap structures in late polyoma virus RNA.多瘤病毒晚期RNA中的多个5'末端帽结构。
Cell. 1979 Feb;16(2):357-71. doi: 10.1016/0092-8674(79)90012-6.
3
A suboptimal 5' splice site is a cis-acting determinant of nuclear export of polyomavirus late mRNAs.次优5'剪接位点是多瘤病毒晚期mRNA核输出的顺式作用决定因素。
Mol Cell Biol. 1996 Nov;16(11):6046-54. doi: 10.1128/MCB.16.11.6046.
4
Polyoma virus-specific RNA synthesis in an inducible line of polyoma virus-transformed rat cells.多瘤病毒转化的大鼠细胞诱导系中的多瘤病毒特异性RNA合成
J Virol. 1978 Mar;25(3):719-29. doi: 10.1128/JVI.25.3.719-729.1978.
5
Amplification in the leader sequence of late polyoma virus mRNAs.多瘤病毒晚期mRNA前导序列中的扩增。
Cell. 1979 Feb;16(2):373-88. doi: 10.1016/0092-8674(79)90013-8.
6
Efficiency of processing of viral RNA during the early and late phases of productive infection by polyoma virus.多瘤病毒在生产性感染的早期和晚期阶段对病毒RNA的加工效率。
J Virol. 1981 Feb;37(2):628-35. doi: 10.1128/JVI.37.2.628-635.1981.
7
Strand-specific transcription of polyoma virus DNA-early in productive infection and in transformed cells.多瘤病毒DNA的链特异性转录——在生产性感染早期和转化细胞中。
J Virol. 1975 Jan;17(1):20-6. doi: 10.1128/JVI.17.1.20-26.1976.
8
Polyoma virus early and late mRNAs in productively infected mouse 3T6 cells.多瘤病毒在高效感染的小鼠3T6细胞中的早期和晚期信使核糖核酸
J Virol. 1982 Oct;44(1):175-88. doi: 10.1128/JVI.44.1.175-188.1982.
9
Nuclear conversion of microinjected avian leukosis virion RNA into an envelope-glycoprotein messenger.
Nature. 1978 Jun 29;273(5665):779-82. doi: 10.1038/273779a0.
10
Location of the sequences coding for capsid proteins VP1 and VP2 on polyoma virus DNA.
Cell. 1976 Nov;9(3):481-7. doi: 10.1016/0092-8674(76)90093-3.

引用本文的文献

1
Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: colinear and interrupted mRNAs code for overlapping proteins.源自流感病毒基因组RNA片段7的mRNA序列:共线性和间断性mRNA编码重叠蛋白。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4170-4. doi: 10.1073/pnas.78.7.4170.
2
Kinetics and efficiency of polyadenylation of late polyomavirus nuclear RNA: generation of oligomeric polyadenylated RNAs and their processing into mRNA.多瘤病毒晚期核RNA的多聚腺苷酸化动力学及效率:寡聚多聚腺苷酸化RNA的产生及其加工成mRNA的过程
Mol Cell Biol. 1984 Apr;4(4):722-9. doi: 10.1128/mcb.4.4.722-729.1984.
3
Adenovirus type 2 fiber mRNA synthesis: no evidence for a cytoplasmic processing pathway.
腺病毒2型纤维mRNA的合成:无细胞质加工途径的证据。
J Virol. 1982 Oct;44(1):226-34. doi: 10.1128/JVI.44.1.226-234.1982.
4
Spliced and unspliced virus specific RNA sequences are associated with purified simian virus 40 chromatin.剪接和未剪接的病毒特异性RNA序列与纯化的猿猴病毒40染色质相关。
Nucleic Acids Res. 1982 Aug 11;10(15):4543-50. doi: 10.1093/nar/10.15.4543.
5
Efficiency of processing of viral RNA during the early and late phases of productive infection by polyoma virus.多瘤病毒在生产性感染的早期和晚期阶段对病毒RNA的加工效率。
J Virol. 1981 Feb;37(2):628-35. doi: 10.1128/JVI.37.2.628-635.1981.
6
Electron microscopic demonstration of the presence of amplified sequences at the 5'-ends of the polyoma virus late mRNAs.用电镜证明多瘤病毒晚期mRNA 5′端存在扩增序列。
Nucleic Acids Res. 1980 Apr 11;8(7):1505-19. doi: 10.1093/nar/8.7.1505.
7
Simian virus 40 cRNA is processed into functional mRNA in microinjected monkey cells.猿猴病毒40的互补RNA(cRNA)在显微注射的猴细胞中被加工成功能性信使核糖核酸(mRNA)。
EMBO J. 1982;1(9):1081-8. doi: 10.1002/j.1460-2075.1982.tb01300.x.