一种小鼠睾丸特异性转录本（ferT）编码一种截短的Fer蛋白。
A murine fer testis-specific transcript (ferT) encodes a truncated Fer protein.
作者信息
Fischman K, Edman J C, Shackleford G M, Turner J A, Rutter W J, Nir U
机构信息
Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
出版信息
Mol Cell Biol. 1990 Jan;10(1):146-53. doi: 10.1128/mcb.10.1.146-153.1990.
Abstract
A cDNA for a potential tyrosine kinase-encoding mRNA was isolated from a mouse testis cDNA library. In a survey of eight mouse tissues, a transcript of 2.4 kilobases restricted to testis tissue was found. The mRNA encodes a 453-amino-acid protein of 51,383 daltons, the smallest tyrosine kinase protein ever described. RNA synthesized from the cDNA template directs the synthesis of a 51,000-Mr protein in a cell-free translation system. The carboxy-terminal 409 amino acids are 98 and 90% identical to the carboxy halves of the rat and human Fer proteins, respectively. This suggests that the cDNA represents an alternatively spliced testis-specific fer mRNA and is therefore termed by us ferT. On the basis of the appearance time of the fer mRNA in the testis of maturing neonatal mice, we speculate on the role played by this protein in the development of this organ.
摘要
从一个小鼠睾丸cDNA文库中分离出了一个可能编码酪氨酸激酶的mRNA的cDNA。在对八种小鼠组织的调查中，发现了一个2.4千碱基的转录本，该转录本仅存在于睾丸组织中。该mRNA编码一个由453个氨基酸组成、分子量为51383道尔顿的蛋白质，这是迄今所描述的最小的酪氨酸激酶蛋白。从cDNA模板合成的RNA在无细胞翻译系统中指导合成一个分子量为51000的蛋白质。该蛋白的羧基末端409个氨基酸分别与大鼠和人类Fer蛋白的羧基末端部分有98%和90%的同源性。这表明该cDNA代表了一个选择性剪接的睾丸特异性fer mRNA，因此我们将其命名为ferT。根据fer mRNA在新生小鼠睾丸中出现的时间，我们推测了该蛋白在这个器官发育过程中所起的作用。
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