Department of Medical Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150081, China.
Carbohydr Polym. 2012 Nov 6;90(4):1671-6. doi: 10.1016/j.carbpol.2012.07.048. Epub 2012 Jul 27.
Two acidic polysaccharide fractions (PTPa and PTPb) extracted from the roots of Polygala tenuifolia, were obtained by DEAE-Sephacel anion-exchange, and Sephadex G-100 gel-permeation chromatography. High-performance liquid chromatography (HPLC) identified that PTPa and PTPb was composed of Ara, Glc, Gal, Man and GlcUA in the proportion of 2.4:1.2:0.6:0.4:1.1 and 2.1:1.7:0.5:0.6:1.7, respectively. Their molecular weight was evaluated to be 5.9×10(4) (PTPa) and 2.5×10(4) Da (PTPb) as determined by high performance size exclusion chromatography (HPSEC). Pharmacological studies revealed PTPa and PTPb significantly inhibited the growth of A549 cells in vitro and exhibited significantly higher antitumor activity against solid tumor A549 in vivo than did a blank control. Moreover, treatment with two acidic polysaccharides caused an enhancement of superoxide dismutase (SOD) and catalase (CAT) activities in tumor-bearing mice and a reduction in thiobarbituric acid reactive substances (TBARS) level. Taken together, these results indicated that two acidic polysaccharides from the roots of P. tenuifolia may be useful as potent antitumor agents for the prevention of lung tumorigenesis.
从远志根中提取的两种酸性多糖（PTPa 和 PTPb）通过 DEAE-Sephacel 阴离子交换和 Sephadex G-100 凝胶渗透色谱法得到。高效液相色谱（HPLC）鉴定表明，PTPa 和 PTPb 由 Ara、Glc、Gal、Man 和 GlcUA 组成，比例分别为 2.4:1.2:0.6:0.4:1.1 和 2.1:1.7:0.5:0.6:1.7。通过高效体积排阻色谱（HPSEC）评估，它们的分子量分别为 5.9×10(4)（PTPa）和 2.5×10(4) Da（PTPb）。药理研究表明，PTPa 和 PTPb 显著抑制 A549 细胞在体外的生长，并表现出比空白对照组更高的体内实体瘤 A549 抗肿瘤活性。此外，两种酸性多糖处理可增强荷瘤小鼠中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性，并降低丙二醛（TBARS）水平。综上所述，这些结果表明，远志根中的两种酸性多糖可能是预防肺癌发生的有效抗肿瘤药物。
