Department of Digestive Interventional Radiology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an 710032, China.
Carbohydr Polym. 2013 Jan 30;92(1):479-83. doi: 10.1016/j.carbpol.2012.09.061. Epub 2012 Oct 2.
In this study, we purified and characterized a polysaccharide (SMP-W1) from Salvia miltiorrhiza and investigated its anticancer and immunoregulatory potential in vitro and in vivo. The monosaccharide composition, protein content, uronic acid content, total carbohydrate content, viscosity and molecular weight of SMP-W1 were analyzed. In vitro, SMP-W1 had an antiproliferative effect on hepatocellular carcinoma H22 cells, especially at the high concentration of 400 μg/ml. Simultaneously the polysaccharide SMP-W1 significantly inhibited tumor growth and increased serum superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities in rats, as well as the secretion of TNF-α. In addition, the body weight, spleen index and thymus index in tumor-bearing mice were significantly improved by SMP-W1 treatment. Taken together, these results indicated that SMP-W1 possessed strong in vivo and in vitro anti-tumor activity and improves the immune response in tumor-bearing mice. Therefore, it could be developed as an anti-tumor agent with immunomodulatory activity.
在这项研究中，我们从丹参中纯化和表征了一种多糖（SMP-W1），并研究了其在体外和体内的抗癌和免疫调节潜力。分析了 SMP-W1 的单糖组成、蛋白质含量、糖醛酸含量、总碳水化合物含量、粘度和分子量。体外实验表明，SMP-W1 对肝癌 H22 细胞具有增殖抑制作用，尤其是在 400μg/ml 的高浓度下。同时，多糖 SMP-W1 显著抑制大鼠肿瘤生长，提高血清中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性，以及 TNF-α 的分泌。此外，SMP-W1 处理还显著改善了荷瘤小鼠的体重、脾脏指数和胸腺指数。综上所述，这些结果表明 SMP-W1 具有较强的体内外抗肿瘤活性，并改善了荷瘤小鼠的免疫反应。因此，它可以开发为具有免疫调节活性的抗肿瘤药物。
