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固定化酿酒酵母 Bayanus 于羧甲基纤维素-g-聚(N-乙烯基-2-吡咯烷酮)珠上进行高生产力的生物乙醇发酵。

High productivity bioethanol fermentation by immobilized Saccharomyces bayanus onto carboxymethylcellulose-g- poly(N-vinyl-2-pyrrolidone) beads.

机构信息

Department of Chemistry, Faculty of Sciences and Arts, Kirikkale University, 71450 Yahşihan, Kirikkale, Turkey.

出版信息

Artif Cells Nanomed Biotechnol. 2013 Apr;41(2):137-43. doi: 10.3109/10731199.2012.696069. Epub 2012 Sep 4.

Abstract

Crosslinked graft copolymers of CMC with N-vinyl-2-pyrrolidone (N-VP) were prepared in different grafting yields. Saccharomyces bayanus was immobilized in the graft copolymers of carboxymethylcellulose-g-poly(N-vinyl-2-pyrrolidone) (CMC-g-PVP) for ethanol fermentation. The effects of grafting yield, initial glucose concentration, and crosslinker concentration on the yield of ethanol process was investigated. Ethanol production increased when percentage of N-vinyl-2-pyrrolidone in the graft copolymer was increased. The maximum concentration, productivity, and yield of ethanol were 98.78 g L (-1), 8.23 g L (-1) h (-1) and 0.494 g g (-1), respectively. Reusability of the immobilized yeasts was found that the materials can be used seven times without losing their activity.

摘要

以不同接枝率制备了羧甲基纤维素(CMC)与 N-乙烯基-2-吡咯烷酮(N-VP)的交联接枝共聚物,并将酿酒酵母(Saccharomyces bayanus)固定在接枝共聚物羧甲基纤维素-g-聚(N-乙烯基-2-吡咯烷酮)(CMC-g-PVP)中用于乙醇发酵。考察了接枝率、初始葡萄糖浓度和交联剂浓度对接枝共聚物乙醇发酵产率的影响。当接枝共聚物中 N-乙烯基-2-吡咯烷酮的比例增加时,乙醇的产量增加。乙醇的最大浓度、生产力和产率分别为 98.78 g/L、8.23 g/L/h 和 0.494 g/g。固定化酵母的可重复使用性表明,该材料可以重复使用七次而不损失其活性。

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