College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, China.
J Microbiol Biotechnol. 2013 Apr;23(4):511-7. doi: 10.4014/jmb.1209.09066.
In this work, a fibrous bed bioreactor with high specific surface area and good adsorption efficacy for S. cerevisiae cells was used as the immobilization matrix in the production of ethanol. In batch fermentation, an optimal ethanol concentration of 91.36 g/l and productivity of 4.57 g l(-1) h(-1) were obtained at an initial sugar concentration of 200 g/l. The ethanol productivity achieved by the immobilized cells was 41.93% higher than that obtained from free cells. Ethanol production in a 22-cycle repeated batch fermentation demonstrated the enhanced stability of the immobilized yeast cells. Under continuous fermentation in packed-bed reactors, a maximum ethanol concentration of 108.14 g/l and a productivity of 14.71 g l(-1) h(-1) were attained at 35 degrees C, and a dilution rate of 0.136 h(-1) with 250 g/l glucose.
在这项工作中，我们使用了一种具有高比表面积和良好吸附效果的纤维床生物反应器作为固定化基质，用于生产乙醇。在分批发酵中，初始糖浓度为 200 g/l 时，可获得最佳乙醇浓度为 91.36 g/l，产率为 4.57 g l(-1) h(-1)。固定化细胞的乙醇产率比游离细胞提高了 41.93%。在 22 个循环重复分批发酵中，固定化酵母细胞表现出增强的稳定性。在填充床反应器的连续发酵中，在 35°C 下，葡萄糖浓度为 250 g/l 时，可达到最大乙醇浓度 108.14 g/l 和产率 14.71 g l(-1) h(-1)，稀释率为 0.136 h(-1)。
