Department of Science and Engineering of Oxidic Materials and Nanomaterials, University Politehnica of Bucharest, Bucharest, 011061, Romania.
IEEE Trans Nanobioscience. 2012 Dec;11(4):360-5. doi: 10.1109/TNB.2012.2208474. Epub 2012 Aug 29.
The aim of the present study was to demonstrate that Fe(3)O(4)/oleic acid core/shell nanostructures could be used as systems for stabilizing the Eugenia carryophyllata essential oil (EO) on catheter surface pellicles, in order to improve their resistance to fungal colonization. EO microwave assisted extraction was performed in a Neo-Clevenger (related) device and its chemical composition was settled by GC-MS analysis. Fe(3)O(4)/oleic acid-core/shell nanoparticles (NP) were obtained by a precipitation method under microwave condition. High resolution transmission electron microscopy (HR-TEM) was used as a primary characterization method. The NPs were processed to achieve a core/shell/EO coated-shell nanosystem further used for coating the inner surface of central venous catheter samples. The tested fungal strains have been recently isolated from different clinical specimens. The biofilm architecture was assessed by confocal laser scanning microscopy (CLSM). Our results claim the usage of hybrid nanomaterial (core/shell/coated-shell) for the stabilization of E. carryophyllata EO, which prevented or inhibited the fungal biofilm development on the functionalized catheter, highlighting the opportunity of using these nanosystems to obtain improved, anti-biofilm coatings for biomedical applications.
本研究旨在证明 Fe(3)O(4)/油酸核/壳纳米结构可用作稳定导管表面薄膜中丁香桉叶油（EO）的系统，以提高其抗真菌定植的能力。EO 的微波辅助提取在新型克利夫兰（相关）装置中进行，并通过 GC-MS 分析确定其化学成分。通过微波条件下的沉淀法获得 Fe(3)O(4)/油酸核/壳纳米粒子（NP）。高分辨率透射电子显微镜（HR-TEM）用作主要的表征方法。对 NPs 进行处理，以实现进一步用于涂覆中心静脉导管样品内表面的核/壳/EO 涂壳纳米系统。测试的真菌菌株是最近从不同的临床标本中分离出来的。通过共聚焦激光扫描显微镜（CLSM）评估生物膜结构。我们的结果声称使用混合纳米材料（核/壳/涂壳）来稳定丁香桉叶油 EO，这可以防止或抑制功能化导管上真菌生物膜的形成，突出了使用这些纳米系统获得改进的、抗生物膜涂层的机会，用于生物医学应用。
