Grupo de Microbiología Molecular, Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombia.
Am J Trop Med Hyg. 2012 Nov;87(5):910-20. doi: 10.4269/ajtmh.2012.12-0285. Epub 2012 Sep 4.
Anopheles triannulatus s.l. is a species complex, however in Colombia its taxonomic status is unclear. This study was conducted to understand the level of genetic differentiation or population structure of specimens of An. triannulatus s.l. from northwestern and southeastern Colombia. Cytochrome oxidase subunit I (COI) and internal transcribed spacer (ITS2) sequence analyses suggested high genetic differentiation between the NW and SE populations. A TCS network and Bayesian inference analysis based on 814 bp of COI showed two main groups: group I included samples from the NW and group II samples from the SE. Two main ITS2-polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) patterns were found. Pattern I is present in both the NW and SE, and pattern II is found in the SE specimens. To further elucidate the taxonomic status of An. triannulatus s.l. in Colombia and how these COI lineages are related to the Triannulatus Complex species, the evaluation of immature stages, male genitalia, and additional mitochondrial and nuclear markers will be needed.
三带喙库蚊复合体在哥伦比亚的分类地位尚不清楚。本研究旨在了解哥伦比亚西北部和东南部三带喙库蚊复合体标本的遗传分化或种群结构水平。细胞色素氧化酶亚基 I（COI）和内部转录间隔区（ITS2）序列分析表明，西北和东南种群之间存在高度的遗传分化。基于 COI 814 个碱基的 TCS 网络和贝叶斯推断分析显示出两个主要群体：第一组包括来自西北的样本，第二组包括来自东南的样本。发现了两种主要的 ITS2-聚合酶链反应-限制性片段长度多态性（PCR-RFLP）模式。模式 I 存在于西北和东南，模式 II 存在于东南标本中。为了进一步阐明哥伦比亚三带喙库蚊复合体的分类地位，以及这些 COI 谱系与三带喙库蚊复合体物种的关系，需要评估幼虫期、雄性生殖器以及其他线粒体和核标记。
