Department of Histology and Embryology, Nicolaus Copernicus University, Bydgoszcz, Poland.
Cell Biol Int. 2012;36(12):1129-35. doi: 10.1042/CBI20120274.
Jurkat human lymphoblastoid cells were incubated in increasing concentrations of doxorubicin (0.05, 0.1 and 0.15 μM) to induce cell death, and their expression of cyclin A, B1 and D1 was evaluated by flow cytometry (cell cycle progression, Annexin V assay, percentages and levels of each of the cyclins), transmission electron microscopy (ultrastructure) and confocal fluorescence microscopy (expression and intracellular localization of cyclins). After low-dose doxorubicin treatment, Jurkat cells responded mainly by G2/M arrest, which was related to increased cyclin B1, A and D1 levels, a low level of apoptosis and/or mitotic catastrophe. The influence of doxorubicin on levels and/or localization of selected cyclins was confirmed, which may in turn contribute to the G2/M arrest induced by the drug.
Jurkat 人淋巴母细胞在不同浓度阿霉素（0.05、0.1 和 0.15 μM）中孵育以诱导细胞死亡，并通过流式细胞术（细胞周期进程、Annexin V 测定、各细胞周期蛋白的百分比和水平）、透射电子显微镜（超微结构）和共聚焦荧光显微镜（细胞周期蛋白的表达和细胞内定位）评估其 cyclin A、B1 和 D1 的表达。在低剂量阿霉素处理后，Jurkat 细胞主要通过 G2/M 期阻滞来响应，这与 cyclin B1、A 和 D1 水平升高、低水平的细胞凋亡和/或有丝分裂灾难有关。阿霉素对选定细胞周期蛋白的水平和/或定位的影响得到了证实，这反过来可能有助于药物诱导的 G2/M 期阻滞。
