Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Çapa, Istanbul, Turkey.
Int Immunopharmacol. 2012 Dec;14(4):349-52. doi: 10.1016/j.intimp.2012.08.010. Epub 2012 Aug 28.
Although prooxidant and antioxidant status were reported to be changed in clinical and experimental hypothyroidism, obtained results are conflicting. In addition, in subclinical hypothyroidism, scarced and controversial data are available about oxidative stress. Therefore, we aimed to investigate prooxidant-antioxidant status only in Hashimoto's thyroiditis (HT) patients with subclinical (sHT) and overt hypothyroidism (oHT).
Thirty sHT and 18 oHT patients and 30 healthy control subjects were included in the study. Endogenous and prooxidant 2,2'-azobis-(2-amidinopropane) hydrochloride (AAPH)-induced malondialdehyde (MDA), diene conjugate (DC), protein carbonyl (PC) and nitrotyrosine (NT) levels as well as ferric reducing antioxidant power (FRAP) were determined in serum. In addition, endogenous DC and copper-induced MDA levels were measured in low density lipoprotein (LDL) fraction.
Although there were no significant difference in serum endogenous MDA and DC levels, AAPH-induced MDA levels were significantly increased in sHT patients. All these parameters increased in oHT patients. Serum PC levels were detected to be increased in both sHT and oHT patients. Serum FRAP values did not alter in sHT patients, but they lowered in oHT patients. Endogenous DC and copper-induced MDA levels in LDL fraction did not change in sHT patients. However, these parameters were detected to be increased significantly in oHT patients as compared to controls and sHT patients.
In conclusion, there were significant increases in oxidative stress parameters in serum and LDL-fraction in oHT patients. However, oxidative stress was detected to stimulate partly in serum, but not LDL fraction in sHT patients.
尽管已报道在临床和实验性甲状腺功能减退症中存在促氧化剂和抗氧化剂状态的改变，但所得结果存在冲突。此外，在亚临床甲状腺功能减退症中，关于氧化应激的可用数据很少且存在争议。因此，我们旨在仅研究桥本甲状腺炎（HT）患者亚临床（sHT）和显性甲状腺功能减退症（oHT）中的促氧化剂-抗氧化剂状态。
本研究纳入了 30 例 sHT 患者、18 例 oHT 患者和 30 名健康对照者。测定血清中内源性和促氧化剂 2,2'-偶氮双（2-脒基丙烷）盐酸盐（AAPH）诱导的丙二醛（MDA）、二烯共轭物（DC）、蛋白羰基（PC）和硝基酪氨酸（NT）水平以及铁还原抗氧化能力（FRAP）。此外，还测定了低密度脂蛋白（LDL）部分中的内源性 DC 和铜诱导的 MDA 水平。
尽管 sHT 患者血清内源性 MDA 和 DC 水平无显著差异，但 AAPH 诱导的 MDA 水平显著升高。所有这些参数在 oHT 患者中均升高。sHT 和 oHT 患者的血清 PC 水平均升高。sHT 患者的血清 FRAP 值没有改变，但 oHT 患者的 FRAP 值降低。sHT 患者的 LDL 部分中内源性 DC 和铜诱导的 MDA 水平没有变化，但与对照组和 sHT 患者相比，oHT 患者的这些参数显著升高。
总之，oHT 患者的血清和 LDL 部分中的氧化应激参数显著增加。然而，在 sHT 患者中，氧化应激在血清中部分刺激，但在 LDL 部分中未刺激。
