一种通过新霉素结合增强荧光团-适体对 RNA 标记的结合亲和力的策略。
A strategy to enhance the binding affinity of fluorophore-aptamer pairs for RNA tagging with neomycin conjugation.
机构信息
Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University, Stanford, California 94305-5484, USA.
出版信息
Chem Commun (Camb). 2012 Oct 14;48(80):10034-6. doi: 10.1039/c2cc34498j.
Abstract
Fluorogenic sulforhodamine-neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250-400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores.
摘要
荧光素-sulforhodamine-新霉素缀合物已被设计和合成用于 RNA 标记。缀合物通过与 RNA 适体结合而被荧光激活，并表现出相对于相应未缀合荧光团的结合亲和力增强大于 250-400 倍。
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