Liu Haixiang, Wang Zhidong, Zhang Xueqing, Qiao Yulei, Wu Shengming, Dong Fangting, Chen Ying
Anhui Medical University, Hefei 230032, China.
Radiat Prot Dosimetry. 2013 Apr;154(1):9-17. doi: 10.1093/rpd/ncs138. Epub 2012 Sep 5.
In the study, gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) techniques coupled with principal components analysis (PCA) were used to investigate metabolite perturbations in the serum of the rats exposed to 0.75, 3 or 8 Gy gamma rays. Male standard deviation rats were gamma-irradiated at doses of 0.75, 3 and 8 Gy (1.9 Gy min(-1)) or sham-irradiated. Serum samples were collected over the first 24 h under the exposure to irradiation in order to analyse the samples by GC/TOFMS. And multivariate data were analysed by PCA. The composition of metabolites in serum yielded distinct metabolomic phenotypes for 0.75, 3 and 8 Gy at 24 h after irradiation. Nine serum metabolites were significantly altered as a result of radiation exposure. Up-regulated metabolites included inositol, serine, lysine, glycine, threonine and glycerol; down-regulated metabolites included isocitrate, gluconic acid and stearic acid. The nine metabolites were significantly altered after ionising radiation for they may be the potential biomarkers for the diagnosis of radiation injury.
在该研究中，采用气相色谱/飞行时间质谱联用技术（GC/TOFMS）并结合主成分分析（PCA），来研究暴露于0.75、3或8 Gyγ射线的大鼠血清中的代谢物扰动情况。将雄性标准差大鼠分别以0.75、3和8 Gy（1.9 Gy min⁻¹）的剂量进行γ射线照射或假照射。在照射暴露的前24小时内采集血清样本，以便通过GC/TOFMS分析样本。并通过PCA对多变量数据进行分析。照射后24小时，血清中代谢物的组成在0.75、3和8 Gy剂量下呈现出明显不同的代谢组学表型。由于辐射暴露，九种血清代谢物发生了显著变化。上调的代谢物包括肌醇、丝氨酸、赖氨酸、甘氨酸、苏氨酸和甘油；下调的代谢物包括异柠檬酸、葡萄糖酸和硬脂酸。这九种代谢物在电离辐射后发生了显著变化，因为它们可能是诊断辐射损伤的潜在生物标志物。
