Departament of Food Technology, CeRTA-TPV, Escola Tècnica Superior d' Enginyeria Agrària, Universitat de Lleida, Lleida, Spain.
PLoS One. 2012;7(8):e43308. doi: 10.1371/journal.pone.0043308. Epub 2012 Aug 29.
Epidemiological data suggest that plant-derived phenolics beneficial effects include an inhibition of LDL oxidation. After applying a screening method based on 2,4-dinitrophenyl hydrazine-protein carbonyl reaction to 21 different plant-derived phenolic acids, we selected the most antioxidant ones. Their effect was assessed in 5 different oxidation systems, as well as in other model proteins. Mass-spectrometry was then used, evidencing a heterogeneous effect on the accumulation of the structurally characterized protein carbonyl glutamic and aminoadipic semialdehydes as well as for malondialdehyde-lysine in LDL apoprotein. After TOF based lipidomics, we identified the most abundant differential lipids in Cu(++)-incubated LDL as 1-palmitoyllysophosphatidylcholine and 1-stearoyl-sn-glycero-3-phosphocholine. Most of selected phenolic compounds prevented the accumulation of those phospholipids and the cellular impairment induced by oxidized LDL. Finally, to validate these effects in vivo, we evaluated the effect of the intake of a phenolic-enriched extract in plasma protein and lipid modifications in a well-established model of atherosclerosis (diet-induced hypercholesterolemia in hamsters). This showed that a dietary supplement with a phenolic-enriched extract diminished plasma protein oxidative and lipid damage. Globally, these data show structural basis of antioxidant properties of plant-derived phenolic acids in protein oxidation that may be relevant for the health-promoting effects of its dietary intake.
流行病学数据表明，植物源性酚类化合物的有益作用包括抑制 LDL 氧化。在用基于 2,4-二硝基苯肼与蛋白质羰基反应的筛选方法对 21 种不同的植物源性酚酸进行筛选后，我们选择了最具抗氧化能力的酚酸。然后在 5 种不同的氧化体系以及其他模型蛋白中评估了它们的作用。然后使用质谱法，证明了它们对结构特征明确的蛋白质羰基谷氨酸和氨基己二酸半醛以及 LDL 载脂蛋白中的丙二醛-赖氨酸的积累有不均匀的影响。在基于飞行时间的脂质组学分析之后，我们在 Cu(++)孵育的 LDL 中鉴定出了最丰富的差异脂质为 1-棕榈酰基溶血磷脂酰胆碱和 1-硬脂酰基 sn-甘油-3-磷酸胆碱。所选的大部分酚类化合物可防止这些磷脂的积累以及氧化 LDL 引起的细胞损伤。最后，为了在体内验证这些效果，我们评估了在动脉粥样硬化的一种成熟模型（仓鼠饮食诱导的高胆固醇血症）中富含酚类的提取物对血浆蛋白和脂质修饰的影响。这表明富含酚类的饮食补充剂可减少血浆蛋白的氧化和脂质损伤。总体而言，这些数据表明了植物源性酚酸在蛋白质氧化中抗氧化特性的结构基础，这可能与其饮食摄入对健康的促进作用有关。
