Department of Obstetrics and Gynecology, Jeroen Bosch Hospital, 's-Hertogenbosch, The Netherlands.
PLoS One. 2012;7(8):e43637. doi: 10.1371/journal.pone.0043637. Epub 2012 Aug 31.
Women with a history of mainly severe and early onset preeclampsia have an increased risk of future cardiovascular disease. During these complicated pregnancies increased levels of anti-angiogenic factors can be found. We hypothesize that women with a history of severe very early onset preeclampsia still have increased levels of these biomarkers years after this pregnancy, resulting in increased risk for cardiovascular disease.
Twenty women with severe early onset preeclampsia before 24 weeks' gestation, who delivered between 1993-2003 in a tertiary referral centre and twenty matched controls with uncomplicated pregnancies and healthy term infants, were addressed for participation in the study. Venous plasma samples were analyzed for basic fibroblast growth factor (bFGF), placental growth factor (PLGF), soluble fms-like tyrosine kinase-1 (sFlt-1), vascular endothelial growth factor (VEGF), E- and P-selectin, soluble intercellular adhesion molecule-3 (sICAM-3) and thrombomodulin by ELISA.
Sixteen case subjects and 18 control subjects consented participation. The median time interval index pregnancy to study was 9.4 and 9.7 years for cases and controls, respectively. Median levels for cases-controls (p-value) were not different; bFGF: 17.43-11.11 pg/mL (0.33), sFlt-1: 102.98-101.92 pg/ml (0.84), PLGF: 3.57-4.20 pg/mL (0.38), VEGF: 64.05-45.72 pg/mL (0.73), E-selectin: 5.11-4.68 ng/mL (0.20), P-selectin: 85.35-71.69 ng/mL (0.69), sICAM-3: 0.42-0.63 ng/mL (0.41) and Thrombomodulin: 0.92-0.93 ng/mL (0.59).
There were no differences in angiogenic biomarkers between women with a history of severe early onset preeclampsia versus uncomplicated pregnancy almost 10 years later, suggesting that these angiogenic factors will not contribute to the early detection of women at risk for future cardiovascular disease.
有主要严重且早发型子痫前期病史的女性未来发生心血管疾病的风险增加。在这些复杂的妊娠中，可以发现抗血管生成因子水平升高。我们假设，有严重早发型子痫前期病史的女性，在妊娠后数年仍存在这些生物标志物水平升高，从而增加了心血管疾病的风险。
在一家三级转诊中心，1993 年至 2003 年期间，20 名有严重早发型子痫前期（妊娠 24 周前）的女性和 20 名有单纯妊娠和健康足月婴儿的匹配对照组被邀请参加研究。通过 ELISA 分析静脉血浆样本中的碱性成纤维细胞生长因子（bFGF）、胎盘生长因子（PLGF）、可溶性 fms 样酪氨酸激酶-1（sFlt-1）、血管内皮生长因子（VEGF）、E-和 P-选择素、可溶性细胞间黏附分子-3（sICAM-3）和血栓调节蛋白。
16 名病例组和 18 名对照组同意参与。病例组和对照组的中位时间间隔为指数妊娠至研究分别为 9.4 年和 9.7 年。病例组-对照组中位数（p 值）无差异；bFGF：17.43-11.11 pg/mL（0.33），sFlt-1：102.98-101.92 pg/ml（0.84），PLGF：3.57-4.20 pg/mL（0.38），VEGF：64.05-45.72 pg/mL（0.73），E-选择素：5.11-4.68 ng/mL（0.20），P-选择素：85.35-71.69 ng/mL（0.69），sICAM-3：0.42-0.63 ng/mL（0.41）和血栓调节蛋白：0.92-0.93 ng/mL（0.59）。
在妊娠近 10 年后，有严重早发型子痫前期病史的女性与单纯妊娠女性的血管生成生物标志物无差异，表明这些血管生成因子不会有助于早期发现未来心血管疾病风险的女性。
