Nakamura R, Miyasaka K, Kuyama Y, Kitani K
First Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Dig Dis Sci. 1990 Jan;35(1):55-60. doi: 10.1007/BF01537223.
The effects of intraluminal bile on cholecystokinin release and pancreatic exocrine secretion were studied in conscious rats. Since it has been suggested that bile acid may influence pancreatic secretion indirectly by interacting with luminal protease activities, intraduodenal protease activities were eliminated by pancreatic juice diversion accompanied with simultaneous intraduodenal infusion of aprotinin. This treatment resulted in gradual increases in pancreatic juice flow, bicarbonate and protein outputs, and an increase in plasma cholecystokinin levels, reaching plateau levels 2 hr after the start of the treatment. When endogenous bile was excluded from the intestine, the pancreatic secretion and plasma cholecystokinin concentrations further increased. The intraduodenal infusion of sodium taurocholate during bile pancreatic juice diversion inhibited cholecystokinin release, while pancreatic protein output was only transiently decreased. The results indicate that bile in the duodenum directly regulates cholecystokinin release, probably through its major components, bile salts.
在清醒大鼠中研究了肠腔内胆汁对胆囊收缩素释放和胰腺外分泌的影响。由于有人提出胆汁酸可能通过与腔内蛋白酶活性相互作用间接影响胰腺分泌，因此通过胰液引流并同时十二指肠内注入抑肽酶来消除十二指肠内蛋白酶活性。这种处理导致胰液流量、碳酸氢盐和蛋白质输出逐渐增加，血浆胆囊收缩素水平升高，在处理开始后2小时达到平台水平。当肠道内排除内源性胆汁时，胰腺分泌和血浆胆囊收缩素浓度进一步增加。在胆汁胰液引流期间十二指肠内注入牛磺胆酸钠可抑制胆囊收缩素释放，而胰腺蛋白质输出仅短暂下降。结果表明，十二指肠内的胆汁可能通过其主要成分胆盐直接调节胆囊收缩素的释放。
