《甲酸甲酯和 2-甲基丁烷诱发雄性 ICR 小鼠骨髓细胞微核的研究》
A Study of Micronucleus Induction with Methyl Formate and 2-Methylbutane in Bone Marrow Cells of Male ICR Mice.
机构信息
Center for Chemicals Safety and Health, Occupational Safety and Health Research Institute, KOSHA, Daejeon, Korea.
出版信息
Saf Health Work. 2010 Sep;1(1):80-6. doi: 10.5491/SHAW.2010.1.1.80. Epub 2010 Sep 30.
OBJECTIVES
We investigated the genotoxicity of two chemicals, methyl formate and 2-methylbutane, using male ICR mice bone marrow cells for the screening of micronucleus induction. Although these two chemicals have already been tested numerous times, a micronucleus test has not been conducted and the amounts used have recently been increased.
METHODS
7 week male ICR mice were tested at dosages of 250, 500, and 1,000 mg/kg for methyl formate and 500, 1,000, and 2,000 mg/kg for 2-methlybutane, respectively. After 24 hours of oral administration with the two chemicals, the mice were sacrificed and their bone marrow cells were prepared for smearing slides.
RESULTS
As a result of counting the micronucleated polychromatic erythrocyte (MNPCE) of 2,000 polychromatic erythrocytes, all treated groups expressed no statistically significant increase of MNPCE compared to the negative control group. There were no clinical signs related with the oral exposure of these two chemicals.
CONCLUSION
It was concluded that the two chemicals did not induce micronucleus in the bone marrow cells of ICR mice, and there was no direct proportion with dosage. These results indicate that the two chemicals have no mutagenic potential under each study condition.
目的
我们使用雄性 ICR 小鼠骨髓细胞来检测两种化学物质——甲酸甲酯和 2-甲基丁烷的遗传毒性，以筛选其诱导微核的能力。尽管这两种化学物质已经经过多次测试，但尚未进行微核试验，且最近使用的剂量有所增加。
方法
将 7 周龄雄性 ICR 小鼠分别以 250、500 和 1000mg/kg 的剂量口服甲酸甲酯，以 500、1000 和 2000mg/kg 的剂量口服 2-甲基丁烷。两种化学物质口服 24 小时后，处死小鼠并制备骨髓细胞涂片。
结果
统计 2000 个多染红细胞中的微核多染红细胞（MNPCE）的数量，与阴性对照组相比，所有处理组的 MNPCE 均无统计学意义的增加。这两种化学物质没有出现与口服暴露相关的临床症状。
结论
这两种化学物质在 ICR 小鼠的骨髓细胞中没有诱导微核，且与剂量无直接比例关系。这些结果表明，在每种研究条件下，这两种化学物质均没有潜在的致突变性。
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