银桦树皮对耐甲氧西林金黄色葡萄球菌的抗菌活性。

Antibacterial activity of bark of Alnus pendula against methicillin-resistant Staphylococcus aureus.

机构信息

Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Jeonbuk, Republic of South Korea.

出版信息

Eur Rev Med Pharmacol Sci. 2012 Jul;16(7):853-9.

PMID:22953631

Abstract

BACKGROUND AND OBJECTIVES

Methicillin-resistant Staphylococcus aureus (MRSA) infections are a rapidly growing health problem around the globe. Recently, there has been considerable interest in the use of plant materials as an alternative method to control pathogenic microorganisms. In this study we evaluated the antibacterial activity of bark of Alnus pendula against MRSA.

MATERIALS AND METHODS

The MIC determination was done using the microdilution broth method and bacterial growth was determined by measuring optical density using spectrophotometer.

RESULTS

Alnus pendula bark EtOH extract and fractions (F-1, -2, -3 and -4) were investigated against MRSA. The most active fractions (F-3 and F-4) led to the isolation of oregonin (ORE) and hirsutanone (HIR). These compounds were active against MRSA strains with minimum inhibitory concentrations (MICs) ranging from 31.25 to 250 microg/ml MIC and 2 MIC of HIR completely inhibited the growth of MRSA.

CONCLUSIONS

The bark EtOH extract of Alnus Pendula has potent antibacterial activity against MRSA.

摘要

背景与目的

耐甲氧西林金黄色葡萄球菌（MRSA）感染是全球范围内迅速增长的健康问题。最近，人们对植物材料作为控制致病微生物的替代方法产生了浓厚的兴趣。在这项研究中，我们评估了桦木皮对 MRSA 的抗菌活性。

材料与方法

采用微量稀释肉汤法测定 MIC 值，通过分光光度计测量光密度来确定细菌生长情况。

结果

对桦木皮的乙醇提取物和馏分（F-1、-2、-3 和-4）进行了抗 MRSA 活性的研究。最具活性的馏分（F-3 和 F-4）导致了对奥瑞因（ORE）和毛蕊花酮（HIR）的分离。这些化合物对 MRSA 菌株具有活性，最低抑菌浓度（MIC）范围为 31.25 至 250μg/ml MIC，HIR 的 2MIC 完全抑制了 MRSA 的生长。